Instrument platforms for nano liquid chromatography

0449307 - ÚIACH 2016 RIV NL eng J - Článek v odborném periodiku
Šesták, Jozef - Moravcová, Dana - Kahle, Vladislav
Instrument platforms for nano liquid chromatography.
Journal of Chromatography A. Roč. 1421, NOV (2015), s. 2-17. ISSN 0021-9673. E-ISSN 1873-3778
Grant CEP: GA MV VG20112015021
Institucionální podpora: RVO:68081715
Klíčová slova: nano liquid chromatography * splitless gradient generation * nano LC platforms
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.926, rok: 2015

tThe history of liquid chromatography started more than a century ago and miniaturization and automa-tion are two leading trends in this field. Nanocolumn liquid chromatography (nano LC) and largelysynonymous capillary liquid chromatography (capillary LC) are the most recent results of this processwhere miniaturization of column dimensions and sorbent particle size play crucial role. Very interestingresults achieved in the research of extremely miniaturized LC columns at the end of the last centurylacked distinctive raison d’être and only advances in mass spectrometry brought a real breakthrough.Configuration of nano LC-electrospray ionization mass spectrometry (LC-ESI-MS) has become a basictool in bioanalytical chemistry, especially in proteomics. This review discusses and summarizes past andcurrent trends in the realization of nano liquid chromatography (nano LC) platforms. Special attention isgiven to the mobile phase delivery under nanoflow rates (isocratic, gradient) and sample injection to thenanocolumn. Available detection techniques applied in nano LC separations are also briefly discussed.We followed up the key themes from the original scientific reports over gradual improvements up to thecontemporary commercial solutions.
Trvalý link: http://hdl.handle.net/11104/0250900