biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 63:144-152, 2019 | DOI: 10.32615/bp.2019.017

Characterisation of Arabidopsis flotillins in response to stresses

K. Kroumanová1, D. Kocourková1, M. Daněk1,2, L. Lamparová1,3, R. Pospíchalová1, K. Malínská1, Z. Krčková1, L. Burketová1, O. Valentová3, J. Martinec1, M. Janda1,3,*
1 Institute of Experimental Botany of the Czech Academy of Sciences, CZ-16502, Prague, Czech Republic
2 Department of Experimental Plant Biology, Faculty of Science, Charles University, CZ-12844 Prague, Czech Republic
3 Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, CZ-16628 Prague, Czech Republic

Plant flotillins, a subgroup of the SPFH domain protein superfamily, consist of three proteins, AtFLOT1, AtFLOT2, and AtFLOT3 in Arabidopsis thaliana. The exact functions of flotillins in plant cell has not been established yet. In this study we focused on the role of flotillins in response to both abiotic and biotic stresses and on the response to phytohormones abscisic acid and 1-naphthalene acetic acid (NAA) in A. thaliana. We observed transcriptomic changes of AtFLOT genes in response to high salinity and cold, treatment with 22-amino acid peptide from N-terminal part of flagellin (flg22), and after infection with Botrytis cinerea. Transcription of AtFLOT2 increased up to 60 times after flg22 treatment. Also, treatment with B. cinerea increased transcription of AtFLOT1 10 times and of AtFLOT3 14 times. Furthermore, we used T-DNA knock-out single mutants for all three A. thaliana flotillins and we measured root growth in response to high salinity, cold, phosphate starvation, nitrogen starvation, and abscisic acid and NAA treatments. Subsequently, we measured the reactive oxygen species production and callose accumulation after the treatment with flg22. Next, we performed resistance assays to Pseudomonas syringae pv. tomato DC3000 and B. cinerea. In contrast to transcriptomic changes, knocking-out of only single FLOT gene did not lead to significant changes in response to all tested stresses.

Keywords: abscisic acid, auxin, Botrytis cinerea, callose, cold, nutrient starvation, Pseudomonas syringae, ROS, salinity

Accepted: November 15, 2018; Prepublished online: November 15, 2018; Published online: January 19, 2019  Show citation

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Kroumanová, K., Kocourková, D., Daněk, M., Lamparová, L., Pospíchalová, R., Malínská, K., ... Janda, M. (2019). Characterisation of Arabidopsis flotillins in response to stresses. Biologia plantarum, 63, Article 144-152. https://doi.org/10.32615/bp.2019.017
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