MMSL 2021, 90(3):120-136 | DOI: 10.31482/mmsl.2021.012

MOLECULAR CHARACTERIZATION OF ALCOHOL–ETHER EXTRACT FROM BOVINE TISSUEOriginal article

Klara Kubelkova ORCID...1*, Martin Hubalek ORCID...2, Pavel Rehulka ORCID...1, Helena Rehulkova ORCID...3, David Friedecky ORCID...4, Jitka Zakova ORCID...1, Ales Macela ORCID...1
1 Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
2 Institute of Organic Chemistry and Biochemistry of the CAS, Prague, Czech Republic
3 Faculty of Science, University of Hradec Kralove, Czech Republic
4 Institute of Molecular and Translational Medicine, Palacky University, Olomouc, Czech Republic

It is usual for information to be unavailable regarding the molecular composition of extracts from herbs or animal tissues that are popular in folk medicine. Here, we present analysis of the alcohol–ether extract from bovine tissue analogous to the basic substance used in such commercial products as Retisin, Imuregen, Actovegin, and Solcoseryl. The tested extract contains a whole spectrum of free amino acids, small proteins and oligopeptides of molecular weight up to 10 kDa, various nucleotides, and a small amount of phospholipids. Among the molecules that can explain some biological activities of the extract were identified those of taurine (2-aminoethanesulfonic acid, a derivative of the amino acid cysteine), several defensins, and bactericidal hemoglobin fragments known as hemocidins. All those molecules identified are natural components of bovine tissues, and a substantial number of them might be biologically active in vivo. Others are sources of readily available nutrients.

Keywords: Key words: bovine tissue extract; Juvenil; molecular composition; psychobiotics; biological response modifier; antimicrobial peptides; defensin; hemocidin

Received: March 16, 2021; Revised: March 16, 2021; Accepted: April 7, 2021; Prepublished online: April 22, 2021; Published: September 3, 2021  Show citation

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Kubelkova, K., Hubalek, M., Rehulka, P., Rehulkova, H., Friedecky, D., Zakova, J., & Macela, A. (2021). MOLECULAR CHARACTERIZATION OF ALCOHOL–ETHER EXTRACT FROM BOVINE TISSUE. MMSL, 90(3), 120-136. doi: 10.31482/mmsl.2021.012
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