biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 63:662-670, 2019 | DOI: 10.32615/bp.2019.134

Analysis of apple epidermis in respect to ontogenic resistance against Venturia inaequalis

I. ZAJÍCOVÁ1, E. TIHLAŘÍKOVÁ2, P. CIFROVÁ1, P. KYJAKOVÁ3, V. NEDĚLA2, J. SECHET4, L. HAVELKOVÁ1, J. KLOUTVOROVÁ5, K. SCHWARZEROVÁ1,*
1 Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Prague, Czech Republic
2 Institute of Scientific Instruments, Czech Academy of Sciences, 61264 Brno, Czech Republic
3 Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, 16610 Prague, Czech Republic
4 Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, 78000 Versailles, France
5 Research and Breeding Institute of Pomology, 50801 Holovousy, Czech Republic

In order to understand mechanisms of ontogenic resistance to apple scab, we analyzed various aspects of young and old leaves. We have introduced an apple plants cultivation system where in vitro propagated and rooting explants produce a genetically uniform population of apple (Malus domestica cv. Idared) plants. In this work, we demonstrate that apple plants produced in our cultivation system showed susceptibility to Venturia inaequalis, the cause of apple scab disease in young leaves and resistance in old leaves, which is similar to orchard situation. Our analysis shows that the cessation of epidermal cell expansion and shape formation coincided with the onset of ontogenic resistance in older leaves. Formation of specific cuticular lamellar structures did not coincide with ontogenic resistance onset. Further, chemical composition analysis of wax from young susceptible and old resistant leaves did not reveal specific compounds involved in ontogenic resistance. Differences in homogalacturonan content in cell walls in susceptible and resistant cells as well as decreased methylesterification of pectin in resistant leaves suggest that polysaccharide composition of the cell wall may play a role in mycelium growth and nutrition.

Keywords: apple scab, cell wall, cuticle, homogalacturonan, Malus domestica, pectin.

Received: September 7, 2018; Revised: December 7, 2018; Accepted: January 22, 2019; Published online: October 25, 2019  Show citation

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ZAJÍCOVÁ, I., TIHLAŘÍKOVÁ, E., CIFROVÁ, P., KYJAKOVÁ, P., NEDĚLA, V., SECHET, J., ... SCHWARZEROVÁ, K. (2019). Analysis of apple epidermis in respect to ontogenic resistance against Venturia inaequalis. Biologia plantarum, 63, Article 662-670. https://doi.org/10.32615/bp.2019.134
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