Abstract
We evaluated whether changes in fine root non-structural carbohydrate reserves of Fagus sylvatica and Pinus sylvestris trees influence potential enzymatic activities of their ectomycorrhizal symbionts from winter towards spring reactivation, and whether these changes influence potential soil enzymatic activities. We analyzed sugar and starch concentrations in the fine roots of Fagus sylvatica and Pinus sylvestris and potential activities of ß-glucosidase, ß-xylosidase, and cellobiohydrolase (as proxies for carbon-degrading enzymes) as well as leucine aminopeptidase and chitinase (as proxies for nitrogen-degrading enzymes) of their dominant ectomycorrhizal symbionts as well as in the soil. Sugar concentrations in the fine roots were significantly positively correlated with enzymatic activities of the ectomycorrhizal symbionts. In Pinus sylvestris, both carbon- and nitrogen-degrading enzyme activities showed significant positive correlations with fine root sugar concentrations. In Fagus sylvatica, fine root sugar concentrations were explicitly positively correlated with the activity of nitrogen-degrading enzymes. The chitinase activity in the soil was found to be strongly positively correlated with the enzymatic activity of the ectomycorrhizal symbionts as well as with fine root sugar concentrations. Fine root carbohydrate concentrations of Fagus sylvatica and Pinus sylvestris trees and enzymatic activities of their associated ectomycorrhizal fungi are connected. The specific nutrient demand of the tree species during spring reactivation may affect ectomycorrhizal enzymatic activity via carbon mobilization in the fine roots of Fagus sylvatica and Pinus sylvestris. Moreover, our results suggest that trees indirectly contribute to the degradation of fungal necromass by stimulating ectomycorrhizal chitinase activity in the soil.
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Introduction
Ectomycorrhizal (EM) fungi are pivotal drivers of biogeochemical processes in temperate and boreal forest ecosystems. Ectomycorrhizas can facilitate the majority of nitrogen (N) and phosphorus (P) uptake in exchange for photosynthetically derived carbon (C) from the host plant (Simard et al. 2003; Smith and Read 2008; van der Heijden et al. 2008). EM fungi produce a wide variety of extracellular enzymes that enables them to acquire nutrients from soil organic matter (SOM) (Pritsch and Garbaye 2011). For example, they produce peptidases and chitinases to acquire N from peptides and chitin or phosphatases to cleave organic P residues (Rineau and Courty 2011). In addition, EM fungi possess the ability to produce extracellular enzymes that depolymerize complex C compounds in order to mobilize glucose (Baber et al. 2016; Courty et al. 2007; Courty et al. 2010; Cullings et al. 2008), although this capability varies widely across lineages of EM fungi (Martin et al. 2016). This trait has been associated with potential saprotrophic capabilities of EM fungi (Bödeker et al. 2014; Cullings and Courty 2009; Hobbie et al. 2013; Lindahl and Tunlid 2015; Shah et al. 2016; Talbot et al. 2008), yet several other studies concluded that SOM decomposition by EM fungi is a by-product of the liberation of N (Averill and Hawkes 2016; Cheeke et al. 2017; Trap et al. 2017).
Tree hosts have developed mechanisms to regulate carbohydrate flows towards the fungal partner (Nehls 2008; Nehls et al. 2010) and therefore govern growth and metabolism of EM fungi (Högberg et al. 2010). A recent study by Hupperts et al. (2017) found a significant positive correlation between the amount of sugar in the fine roots and the activity of root invertase. This enzyme hydrolyzes sucrose into glucose and fructose in the interfacial apoplast. As EM fungi lack the genes encoding invertase (Smith and Read 2008), the regulation of invertase activity by the plant may dictate the amount of C allocated to EM fungi (Hupperts et al. 2017). During the growing season, the host supplies its fungal symbionts with recently fixed C (Högberg et al. 2010). However, during times of no or limited photosynthetic activity of the host, the C must either come from non-structural carbohydrate (NSC) reserves of the host (Pringle 2016), or EM fungi may obtain and metabolize C from SOM (Courty et al. 2007; Cullings and Courty 2009; Shah et al. 2016).
Undoubtedly, NSCs constitute a vast and dynamic pool of available C in trees (Körner 2003). NSC stores are mainly composed of different sugars and starch (Fischer and Höll 1991; Hoch et al. 2003) and provide C for anabolic and metabolic processes (Chapin et al. 1990). NSC reserves may also be mobilized during maximum growth in spring and early summer when the C demand exceeds C supply from photosynthesis (Hoch et al. 2003). However, most important are NSC reserves in winter and spring, where they fuel maintenance respiration (Ögren 2000), increase cold tolerance (Wong et al. 2009), and support the growth of new leaves and roots in spring (Bazot et al. 2013; Smith et al. 2017). Therefore, NSC reserves undergo seasonal variations that reflect those source-sink C dynamics. Generally, NSC reserves in temperate tree species are built up during summer when photosynthetic activity is highest and reach its peak at the end of the vegetation period (Barbaroux and Bréda 2002; Furze et al. 2018). During the dormant season, NSC pools of temperate tree species continuously deplete during the winter and were found to be lowest at bud break in spring (Dietze et al. 2014; Schädel et al. 2009).
In order to meet the plants’ specific C demand, NSC reserves can be specifically allocated to, and exchanged between, different plant organs (Bazot et al. 2013; Klein et al. 2016). For example, Smith et al. (2017) suggested that the depletion of NSC reserves in deciduous tree roots in spring was a direct consequence of increased root growth and activity during that time. As plants preferentially channel C to organs responsible for acquiring the growth-limiting resources (Johnson et al. 2013), a sufficient C supply to their mycorrhizal symbionts must have a high priority (Hartmann and Trumbore 2016). Mycorrhizal symbionts are suggested to be a strong C sink (Hartmann and Trumbore 2016; Pringle 2016), and thus, NSC concentrations and C supply to the mycorrhizal fungal partner should be connected. Loewe et al. (2000) analyzed metabolic changes and NSC dynamics of Picea abies seedlings during the formation of mycorrhizal symbiosis with Amanita muscaria and Paxillus involutus. They found significantly increased sucrose phosphate synthase activity and significantly decreased starch concentrations in response to mycorrhiza formation, thus suggesting that increased consumption of C by mycorrhizal symbiosis does relieve C limitation (Loewe et al. 2000). However, the relationship between NSC pools and growth as well as metabolism of EM fungi still remains poorly understood. In particular, this relationship has to our knowledge never been studied during times when supply of recently fixed C by photosynthesis is limited.
Therefore, we set out to investigate the relationship between NSC pools in the fine roots and the potential enzymatic activities of the EM fungal symbionts in a young F. sylvatica and a young P. sylvestris forest stand. We followed the two most abundant EM symbionts in each stand throughout the winter dormancy until bud break (at above-zero soil temperatures), and analyzed the potential enzymatic activities of ß-glucosidase, ß-xylosidase, and cellobiohydrolase as proxies for C-degrading enzymes, and chitinase (N-acetyl-glucosaminidase) and leucine-aminopeptidase as proxies for N-degrading enzymes (Sinsabaugh et al. 2002; Sinsabaugh et al. 2008). We hypothesized that fine root NSC reserves and ectomycorrhizal enzymatic activities are correlated during this period. In addition, we analyzed the potential activity of extracellular enzymes in the soil in order to evaluate whether or rather to which extent plant C allocation may affect the activity of extracellular enzymes in the soil.
Methodology
Site description
The study site is a young temperate forest stand located in Central Burgenland, Austria (47° 22′ 34″ N, 16° 23′ 20″ E, 490 m a.s.l.), under the management of the Esterházy forest holding. According to Pretzsch et al. (2015), mean annual temperature and precipitation is 8.5 °C and 750 mm, respectively. The experiment was conducted on two adjacent, pure, c. 40 year-old Fagus sylvatica and Pinus sylvestris stands. Both plots are approximately 0.2 ha in size and South to Southwest exposed, with a slope inclination of about 35%. The bedrock consists of phyllite- and clay slates. The predominant soil types are Cambisols and Semi-Podzols (IUSS Working Group 2006). pH of the O horizon was 4.6 in the F. sylvatica stand and 4.4 in the P. sylvestris stand. Humus type is a Dys-Moder (Zanella et al. 2011). Five temperature sensors on each plot were installed to hourly record soil temperature at 5 cm depth during the experiment, and moisture content was measured gravimetrically on every sampling date (Fig. 1).
Root/soil sampling and processing
Five sampling dates between end of leaf fall and bud break were chosen: 14.12.2015, 25.1.2016, 7.3.2016, 29.3.2016, and 16.4.2016. The last sampling date was just before bud break, when buds were already swollen and a few leaves were already visible. Within each plot, five representative sampling spots were marked. At each date, one soil core was taken within a circumference of 100 cm of each spot with a stainless steel corer (Ø 80 mm) to a depth of 10 cm. Soil cores were put in styrofoam shells and immediately transported to the laboratory, where they were kept at 3–4 °C. Soil cores were processed within 36 h of sampling. The O-horizon, with an average thickness of c. 2 cm in both stands was chosen for the subsequent soil and root analyses. The O-horizon was carefully separated from the soil core. First, coarse and fine roots were gently separated from the surrounding soil by shaking and the use of a brush. The soil was then passed through a 2 mm sieve and stored at 3–4 °C until further examination. The extracted roots were thoroughly rinsed under tap water and coarse roots (> 2 mm) were disregarded. The fine root (≤ 2 mm) segments were placed on wet tissue in petri dishes and stored at 3–4 °C until further examination.
Morphotyping of ectomycorrhizas
Root segments were examined using a microscope (ZEISS Stemi 2000-CS) connected to an AxioCam ERc5s camera. First, root segments were randomly chosen and 300–400 root tips per sample were subdivided into three fractions: semivital and dead, vital non-mycorrhizal, and vital mycorrhizal root tips. The determination of different ectomycorrhizal morphotypes was based on morphological characteristics as described in Agerer (1987–2002) using macroscopic and microscopic features. Ectomycorrhizas were identified to the genus level using tip color and shape, patch color and frequency, branching pattern, mantle texture and luster, and the extent of the extramatrical hyphae (Agerer 1987–2002). As the morphotypes were not identified to species, we refer to our EM morphotypes as genus + “sp.” (Winston 1999). Matching ectomycorrhizal root tips of the two most abundant EM species were cut at a length of 2–4 mm from the root fragments using a razor blade and stored at 3–4 °C in 2 ml plastic tubes filled with tap water until further examination. After morphotyping, the remaining fine roots were immediately oven-dried for subsequent sugar and starch analyses (see below). The roots were usually dried within 48 h (in an exception case after 60 h) of the initial root extraction form the soils. During the entire processing time, the root samples were kept at 3–4 °C.
Calculation of root tip turnover and decomposition rates
Turnover and decomposition rates of root tips were calculated using a modified decision matrix method (Brunner et al. 2013). Production was determined through increases in vital root tips, death by an increase in non-vital roots tips, and decomposition through the decrease in non-vital roots tips measure over the sampling period.
Root tip enzyme analysis
Potential extracellular enzyme activity (from now on referred to as “EM enzyme activity”) was performed on the dissected ectomycorrhizal root tips according to the protocol of Pritsch et al. (2004) and their improvements in Pritsch et al. (2011) within 48 h of root tip dissection. Potential enzymatic activities are based on the substrates 7-amino-4-methyl coumarin (AMC; for LAP) and 4-methylumbelliferone (MUB; for all others). Substrates for enzymes as well as standard substrates were purchased from Sigma-Aldrich (MO, USA). The following enzyme activities were determined (substrate name, abbreviation, and concentration used in brackets): cellobiohydrolase (4-MUB-ß-D-cellobioside, CEL, 400 μM), ß-glucosidase (4-MUB-ß-D-glucopyranoside, GLU, 500 μM), leucine-aminopeptidase (L-leucine-AMC, LAP, 400 μM), N-acetyl-glucosaminidase (4-MUB-N-acetyl-ß-D-glucosaminide, NAG, 500 μM), and ß-xylosidase (4-MUB-ß-D-xyloside, XYL, 500 μM). Assay pH was 6.5 for LAP and 4.5 for all other substrates. Incubation times ranged between 15 and 70 min.
Previously dissected EM root tips were placed in a 96-well filter plate (AcroPrepTM filter plate, 30–40 μm mesh size, Pall Life Sciences, Crailsheim, Germany) prefilled with 150 μl of rinsing buffer. To start the assay, the rinsing buffer was vacuum-removed and discarded. Immediately after, 100 μl of the first substrate was added to the wells. The filter plate was placed on a horizontal shaker and incubated in the dark for the time of incubation. To stop the enzymatic reaction, black-well plates were prefilled with 150 μl stopping buffer and the substrate was vacuum-transferred into the black-well plate below. Standard curves containing a 50 μM AMC (for LAP) and a 50 μM MUB solution (for all other substrates) were prepared directly on the respective 96-well plates. The plate was closed with a transparent cohesive plastic film and stored in the dark at 20 °C until measurement. The filter plate was flushed with rinsing buffer and the procedure was subsequently repeated with the other substrates. Fluorescence was measured using a Perkin Elmer EnSpire multiplate reader with an excitation of 365 nm and an emission of 450 nm at 20 and 100 flashes. The potential extracellular enzyme activity was calculated using regression curves based on the standard solutions according to Pritsch et al. (2004). Immediately after the enzymatic assay, EM root tips were transferred to a transparent flat-bottom 96-well plate prefilled with 200 μl of water. To determine the surface area, root tips were scanned and analyzed using the image analysis software WinRhizo (Regent Instruments, Québec, Canada). The potential extracellular enzyme activity is expressed in nmol cm−2 h−1.
Sugar and starch analysis
Fine root segments (≤ 2 mm) were oven-dried for 3 days at 60 °C. Immediately after drying, fine roots were ground with a ball mill at low speed and stored in air-tight plastic bags in the dark at room temperature. Measurement of soluble sugar and starch concentrations was carried out following the protocol of Chow and Landhäusser (2004). Briefly, 100 mg of root material were mixed with 5 ml of an 80% ethanol solution and incubated at 95 °C for 10 min. After centrifugation, the supernatant was removed. This extraction was repeated two more times, yielding in 15 ml ethanol containing the soluble sugars. The residues in the tube were stored wet at − 20 °C for starch analysis. Sugar concentrations were measured colorimetrically at 490 nm using phenol-sulfuric acid with a 2% phenol concentration. Sugar concentrations were determined against a glucose standard.
For the determination of starch, the residues were digested for 30 min at 50 °C using 2 ml of a 0.1 M sodium hydroxide solution. Thereafter, the solution was neutralized with 2.5 ml of a 0.1 M sodium acetate solution and 0.5 ml of an enzyme mixture containing 400 U ml−1 of α-amylase (ICN Biomedicals, CA, USA) and 2 U ml−1 of amyloglucosidase (Sigma-Aldrich) were added. This mixture was then incubated for 48 h at 50 °C. A peroxidase-glucose oxidase/o-dianisidine reagent (Sigma-Aldrich) was used to measure the amount of hydrolysed glucose colorimetrically at 525 nm. The obtained sugar concentrations were determined against a glucose standard using linear regression curves. The concentrations of sugar and starch are expressed as % of total dry weight.
Soil enzyme analysis
The analysis of potential extracellular enzymatic activities in the soil (from now on referred to as “soil enzyme activity”) was performed within 4 days of sampling according to the method described in Marx et al. (2001), Saiya-Cork et al. (2002), and German et al. (2011). One gram of soil was suspended in 100 ml of a 100 mM sodium acetate buffer, pH 4.5, and homogenized for 1 min in a sonication bath. Aliquots of 200 μl were pipetted under constant stirring on a magnetic stir plate into black 96-well microplates, with four replicates for each sample. Optimal substrate concentrations and incubation times for cellobiohydrolase (CEL, 0.3 mM), ß-glucosidase (GLU, 0.5 mM), leucine-aminopeptidase (LAP, 1 mM), N-acetyl-glucosaminidase (NAG, 1 mM), and ß-xylosidase (XYL, 1 mM) were evaluated ahead (all substrates were purchased from Sigma-Aldrich). Substrates were added to each well and horizontally shaken for 30 s in order to mix the sample suspension with the substrate. The black-well plates were covered with a cohesive plastic film and incubated in the dark at 20 °C. Incubation time was 120 min. A set of standard curves for both AMC (for LAP; 50 μM, 100 μM) and MUB (for all the others; 20 μM, 50 μM, 100 μM, 250 μM) were prepared in order to cover a wide range of fluorescence. Fluorescence was measured using a Perkin Elmer EnSpire multiplate reader with an excitation of 365 nm and an emission of 450 nm at 20 and 100 flashes. The potential enzyme activity is expressed in nmol g dry soil−1 h−1.
Statistical analyses
One-way ANOVA followed by post hoc Tukey tests were used to test whether (i) sugar and starch concentrations, (ii) potential enzymatic activities (n = 10–16) and ratio of C- to N-degrading enzymes of the two dominant EM symbionts, and (iii) potential soil enzymatic activities in each stand differed significantly at each of the five sampling dates (at the p < 0.05 level). One-way ANOVA was also used to test for significant differences between Cenococcum sp. associated with Fagus sylvatica and Cenococcum sp. associated with Pinus sylvestris (p < 0.05). Linear regression analyses were used to test the relationship between (i) sugar concentrations in the fine roots and EM enzymatic activity, (ii) the relationship between soil enzyme and EM enzyme activity, and (iii) the relationship between soil enzyme and the sugar concentration in the fine roots. We refer to a p value of < 0.05 as statistically significant. Complementary, analyses of covariance (ANCOVA) with tree species as the covariate were used to further evaluate the abovementioned relationships (p < 0.05). All statistical tests were performed using the software SPSS statistics 24.
Results
Soil temperature and moisture
Throughout the whole sampling period, the soil temperature in both stands was above 0 °C (Fig. 1). From December 1, 2015, to April 30, 2016, the mean soil temperature in 5-cm depth was 7.0 and 6.8 °C in the Fagus sylvatica and Pinus sylvestris stand, respectively. A greater fluctuation in temperature was shown in the P. sylvestris stand. Soil moisture in the F. sylvatica stand ranged from 33 to 60%, while soil moisture in the P. sylvestris stand ranged from 19 to 41%. Soil moisture was higher under F. sylvatica compared to P. sylvestris at all five sampling dates.
Root properties
A total of 9579 and 7587 root tips were analyzed in the F. sylvatica and the P. sylvestris stand, respectively, during the whole sampling period. The vital fraction of the root tips including mycorrhizal and non-mycorrhizal root tips was 37 and 22% for F. sylvatica and P. sylvestris, respectively, in December 2015 (Online Resource 1). The highest share of vital root tips in the F. sylvatica stand was found in mid-April with 67%, while the highest share of vital root tips in the P. sylvestris stand was found in mid-March with 51%. Mycorrhizal root tips steadily increased from 32% in December 2015 to 67% in April 2016 in the F. sylvatica stand, and from 15 to 46% in the P. sylvestris stand. Root tip turnover was 1.09 a−1 and 1.80 a−1, and root tip decomposition was 1.35 a−1 and 0.97 a−1 for F. sylvatica and P. sylvestris, respectively.
Sugar and starch concentrations in the fine roots
Sugar concentrations in the fine roots of F. sylvatica were lowest in December 2015 and January 2016. Thereafter, sugar levels increased significantly up to 2.7% (± 0.43) of total dry weight at the end of March 2016 (Fig. 2). As for the fine roots in the P. sylvestris stand, sugar concentrations remained low until March 2016, and thereafter significantly increased to 2.4% (± 0.24) of total dry weight in mid-April 2016.
Starch concentrations in the fine roots behaved rather similar for both tree species, i.e., a significant increase from December 2015 towards the beginning of March 2016 followed by a significant decrease towards the fourth and fifth sampling date (Fig. 2).
Ectomycorrhizal abundance and activity
In the F. sylvatica stand, Lactarius sp. and Cenococcum sp. were the two most dominant EM taxa. Both taxa together comprised between 55 and 77% of the total EM community (Fig. 3). As for the P. sylvestris stand, Tylospora sp. and Cenococcum sp. represented the two dominant EM taxa, comprising between 56 and 84% of the total EM community (Fig. 3). Pictures of the EM morphotypes can be found in the Online Resource 2.
Patterns were similar among GLU, XYL, and CEL (from now on referred to as “C-degrading enzymes) as well as among NAG and LAP (from now on referred to as “N-degrading enzymes”) (Tables 1 and 2). GLU activity represented around 60–70% of the total activity of C-degrading enzymes, while NAG activity represented 80–95% of the total activity of N-degrading enzymes (Tables 1 and 2). For reasons of clarity, only the sum activity of all C- and N-degrading enzymatic activities, respectively, are displayed in both Figs. 3 and 4 for the two dominant EM species. Detailed activities of all enzymes can be found in Table 1 and Table 2.
In the F. sylvatica stand, the activity of N-degrading enzymes of Lactarius sp. was lowest in December and significantly increased towards spring reactivation in April 2016. The activity of C-degrading enzymes was highest in January 2016. In early March 2016, the activity significantly dropped, yet rose again in late March 2016 (Table 1, Fig. 3). The ratio of C- to N-degrading enzymes was highest in December 2015 and then steadily and significantly dropped towards spring reactivation. Both C- and N-degrading enzymatic activities of Cenococcum sp. were high in December 2015 and decreased significantly in late January 2016 and early March 2016. Thereafter, both the C- and N-degrading enzymes significantly increased towards spring reactivation. The ratio of C- to N-degrading enzymes dropped steadily and significantly towards spring reactivation (Fig. 3).
In the P. sylvestris stand, activity of both C- and N-degrading enzymes of Tylospora sp. were low from December 2015 until early March 2016 and steadily and significantly increased thereafter towards spring reactivation (Table 2, Fig. 4). The ratio of C- to N-degrading enzymes was highest in December 2015 and decreased significantly towards spring reactivation. The activity of both C- and N-degrading enzymes of Cenococcum sp. in the P. sylvestris stand was lowest in late January and early March 2016 and significantly increased in late April 2016 (Table 2, Fig. 4). The ratio of C- to N-degrading enzymes was not significantly different between all sampling dates.
Throughout the whole sampling period, Cenococcum sp. associated with F. sylvatica showed significantly higher NAG activity as compared to Cenococcum sp. associated with P. sylvestris (p < 0.001). LAP activity of Cenococcum sp. on F. sylvatica was significantly higher in December 2015 and on both sampling dates in early and late March 2016 (p < 0.01) as compared to Cenococcum sp. on P. sylvestris. GLU activity of Cenococcum sp. on F. sylvatica was significantly higher in December 2015 and at the end of March 2016 as compared to Cenococcum sp. on P. sylvestris (p < 0.01). XYL activity of Cenococcum sp. associated with F. sylvatica was significantly higher in December 2015, January 2016 and April 2016 as compared to Cenococcum sp. associated with P. sylvestris (p < 0.05), while CEL activity of Cenococcum sp. on F. sylvatica was significantly higher in December 2015 and at both sampling dates in early and late March 2016 as compared to Cenococcum sp. on P. sylvestris (p < 0.05).
Significantly positive relationships in the F. sylvatica stand were recorded between the fine root sugar concentration and the EM enzymatic activity of both NAG (p = 0.034) and LAP (p = 0.011), yet not for the activity of C-degrading enzymes nor for the ratio of C- to N-degrading enzymes (p = 0.052) (Fig. 5). In the P. sylvestris stand, significantly positive correlations were found between the sugar concentration and EM enyzmatic activities of GLU (p = 0.027), XYL (p = 0.027), CEL (p = 0.036), and NAG (p = 0.029), yet not for LAP (p = 0.063), and a significantly negative correlation with the ratio of C- to N-degrading enzymes (p = 0.047). When an analysis of covariance (ANCOVA) was applied, significant positive relationships between the sugar concentration in fine roots and the activity of LAP (p = 0.001) and NAG (p = 0.006) were detected across both tree species, yet not between GLU, XYL and CEL and fine root sugar concentrations. Moreover, a significantly negative relationship between sugar concentrations and the ratio of C- to N-degrading enzymes was found (p = 0.002).
Potential soil enzymatic activity
The temporal patterns of the soil NAG and LAP activity were similar, as the concentrations increased steadily from December 2015 to April 2016 in both stands (Online Resource 3). GLU activity levels in the soil were low in December 2015 and January 2016 for both stands, and significant increased until early March 2016, after which the activity levels remained constant until April 2016. XYL activity was low in December 2015 and January 2016 in both stands, and increased significantly towards spring reactivation in April 2016. In the P. sylvestris stand, a significant increase in the XYL activity was already recorded in early March 2016. Activity of CEL in both stands did not show significant differences between sampling dates. No significant differences in the ratio of C- to N-degrading enzymes were found in the F. sylvatica and P. sylvestris stands across the whole sampling period.
Soil NAG activity was significantly positively correlated with the NAG activity of the two dominant EM species in both F. sylvatica (p = 0.039) and P. sylvestris (p = 0.04) stands (Fig. 6a). Such a significant relationship was not found for the other enzymes. Moreover, soil NAG activity was significantly positively correlated with the sugar concentration in the fine roots of F. sylvatica (p = 0.002) and P. sylvestris (p = 0.04) (Fig. 6b). GLU activity in the soil was also found to be significantly correlated with the sugar concentrations in the fine roots when both tree species were analyzed together using ANCOVA (R2 = 0.510, p = 0.034). No such relationships were found for LAP, XYL, and CEL.
Discussion
Root properties
Our study clearly shows that the transition period from winter towards spring is a period of reactivation in the soil as well as in the plants and their associated EM fungal symbionts. In general, root colonization by EM fungi is low in winter and increases towards spring, with highest colonization rates in summer (Rastin et al. 1990; Swaty et al. 1998). This pattern was found to be consistent in our study, where root colonization by EM fungi was low in winter and doubled in the Fagus sylvatica stand and tripled in the Pinus sylvestris stand towards spring reactivation. Swaty et al. (1998) suggested that the tree hosts might not be able to support their EM fungal symbionts during winter due to reduced or suppressed photosynthetic rates, thus resulting in low colonization rates during this period.
Enzymatic activity of EM fungi
The two abundant EM taxa in the F. sylvatica and P. sylvestris stand, respectively, displayed a significant increase in both their C- and N-degrading enzymatic activity from January 2016 on towards spring reactivation (except for the C-degrading enzymatic activity of Lactarius sp. in the F. sylvatica stand; see Figs. 3 and 4), a pattern that is consistent with other studies (e.g., Buée et al. 2005; Courty et al. 2007). In general, the enzymatic activity of both EM symbionts was higher in the F. sylvatica stand than in the P. sylvestris stand. This could reflect different belowground C-use strategies of coniferous and deciduous tree species. For example, fine root biomass in F. sylvatica stands is higher than that in P. sylvestris stands in the temperate ecotone (Finér et al. 2007). Moreover, the higher root tip turnover rates of P. sylvestris root tips (1.80 a−1) as compared to F. sylvatica root tips (1.09 a−1) suggest that potentially more C is dedicated towards the production of new roots in P. sylvestris, while F. sylvatica may dedicate more C towards its fungal partner in order to explore the soil matrix and acquire nutrients.
Interestingly, Cenococcum sp. associated with F. sylvatica exhibited significantly higher enzymatic activities as compared to Cenococcum sp. associated with P. sylvestris, although both plots are in close proximity. Enzymatic activities can vary considerably within a species, and variations were often found to be linked to soil characteristics (Courty et al. 2005; Pritsch and Garbaye 2011). Species within the genera Cenococcum can also exhibit a high genetic variability (LoBuglio et al. 1991). For example, Jany et al. (2002) detected four different Cenococcum geophilum genotypes at the stand scale, and up to three genotypes appeared in one single soil core. Moreover, functional attributes can vary considerably between different genotypes (Jany et al. 2003), which could also explain the differences.
Both EM fungi in the F. sylvatica stand significantly increased the production of N-degrading enzymes towards spring reactivation, while the production of C-degrading enzymes did not change. As a result thereof, a significant decrease in the ratio of C- to N-degrading enzymes towards spring reactivation for Lactarius sp. and Cenococcum sp. in F. sylvatica was shown (Fig. 3). A minor shift in the enzymatic activity was also found for Cenococcum sp. in the P. sylvestris stand, yet not for Tylospora sp. (Fig. 4). Therefore, we conclude that there is a shift towards EM-derived NAG and LAP production as compared to GLU, XYL and CEL in the F. sylvatica stand, while resources were more equally distributed among all measured enzymes in the P. sylvestris stand.
Seasonality of non-structural carbohydrate reserves
In agreement with several studies (Barbaroux and Bréda 2002; Chapin et al. 1990; Dietze et al. 2014; Ögren 2000), sugar concentrations in the fine roots of F. sylvatica and P. sylvestris showed the expected seasonal fluctuation during our sampling period, i.e., low sugar levels in winter and a fourfold increase towards spring reactivation (Fig. 2). In contrast, fine root starch concentrations of both tree species peaked in early March and thereafter decreased significantly towards spring reactivation. This clearly indicates a transfer from other reserve organs towards the fine roots (Mei et al. 2015) during early March, and a subsequent conversion to sugar (Richardson et al. 2013; Scartazza et al. 2013; Trumbore et al. 2015), potentially explaining the high fine root sugar concentrations at the succeeding sampling dates in late March and mid-April 2016.
The concentrations of sugar and starch in fine roots are well within the expected range of 2–20% (Barbaroux et al. 2003; Körner 2003) and were found to be rather similar between the fine roots of F. sylvatica and P. sylvestris (Richardson et al. 2015). According to literature, NSC reserves fluctuate stronger in deciduous trees compared to coniferous trees (Kramer and Kozlowski 1979; Piispanen and Saranpää 2001) as more C reserves must be stored and mobilized in order to support leaf flush and initial tree ring formation in spring (Hoch et al. 2003). However, NSC concentrations in fine roots do not seem to underlie such strong fluctuations.
Linking ectomycorrhizal enzyme production and NSC reserves
We hypothesized that Fagus sylvatica and Pinus sylvestris trees influence the enzymatic activity of EM fungal taxa due to alterations in fine root NSC concentrations and subsequently C allocation during spring reactivation. Indeed, we found sugar — yet not starch — concentrations in the fine roots to be significantly positively correlated with EM-derived enzymatic activities (Fig. 5). Potential NAG and LAP activities of the dominant EM taxa were strongly correlated to sugar concentrations in the fine roots of both F. sylvatica and P. sylvestris. Those two enzymes are involved in the mobilization of N from peptides and chitin but can also be important in mobilization of C as well (Sinsabaugh and Follstad Shah 2012). NAG activity represented 80–95% of the total activity of N-degrading enzymes (Pritsch and Garbaye 2011), suggesting that the mobilization of N and potentially C from chitin, a structural polysaccharide that constitutes around 16% of the dry mass of filamentous fungi (Dahiya et al. 2006), seems particularly relevant. The mycelium of ectomycorrhizal fungi is a major contributor to the pool of microbial necromass in terrestrial forest ecosystems (Read and Perez-Moreno 2003). During winter and spring, fungal necromass has been shown to be high compared to later in the season (Ekblad et al. 2013; Wallander et al. 1997; Wallander et al. 2001), likely because tree hosts may not be able to support their EM fungal symbionts during winter due to reduced or suppressed photosynthetic rates (Swaty et al. 1998). Therefore, it seems highly plausible that plant C allocation to the EM symbiont induces the decomposition of fungal necromass via enzymatic activities during spring reactivation (Drigo et al. 2012).
In the P. sylvestris stand, we observed a significant correlation between sugar concentrations in fine roots and the ectomycorrhizal production of N- as well as C-degrading enzymes. In contrast, C supply to EM fungi in F. sylvatica seems to be particularly linked to the degradation of N-rich compounds. Different mechanisms in nutrient cycling and translocation between deciduous and coniferous tree species may account for the observed pattern (Ribbons et al. 2016). Early spring represents a period with particularly strong N demand in deciduous trees due to the establishment of new leaves. Early evidence suggests that much of the N in the leaves in deciduous trees was moved from belowground, while N translocation from the root system to the leaves in coniferous trees occurred gradually throughout the growing season (Chapin et al. 1980; Chapin and Kedrowski 1983). The establishment of new leaves during spring reactivation constitutes a major N-sink and therefore suggests a more pronounced state of N-limitation in deciduous compared to coniferous trees during this period (Marschner 2011), which could explain the observed patterns. Thus, our results substantiate that EM symbioses signify a high priority for their tree hosts, particularly during periods of increased nutrient requirement (Högberg et al. 2003; Zhang et al. 2015).
Relationship between EM enzymatic activities and soil enzymatic activities
Higher plant photosynthetic activity during spring reactivation not only promotes EM fungal activity, but soil microbial activity in general (Kaiser et al. 2010; Žifčáková et al. 2016). All potential C- and N-degrading enzymatic activities except of CEL increased in both forests towards spring reactivation (Rastin et al. 1988). While activities of NAG and LAP increased rather synchronously with sugar concentrations in the fine roots, GLU activities in both forest stands were already high at the beginning of March, suggesting that the activity of this enzyme is a trait of microbial groups other than ectomycorrhizal fungi and rhizosphere bacteria (Kaiser et al. 2010).
The relative and absolute contribution of EM fungi and other microbial groups to enzyme production in soil remains rather ambiguous (Phillips et al. 2014; Talbot et al. 2013). In our study, we found a strong relationship between EM-derived NAG activity and soil NAG activity (Fig. 6a), yet neither LAP nor the C-degrading enzymes showed this relationship. This suggests that EM fungi contribute substantially to the degradation of chitin during spring reactivation and supports earlier studies suggesting that NAG activity is an indicator for fungal activity in general (Hodge et al. 1995; Miller et al. 1998). Moreover, sugar concentrations in the fine roots and NAG activity in the soil were significantly positively correlated in both forest stands (Fig. 6b), revealing a direct and significant link between the host’s C status in the fine roots and the enzymatic activity of microbes in the soil.
Conclusion
In this study, we set out to investigate the relationship between fine root NSC reserves and the enzymatic activity of EM fungal symbionts in a Fagus sylvatica and a Pinus sylvestris stand. We hypothesized that non-structural carbohydrate reserves in the fine roots and the production of C- and N-degrading enzymes of the EM fungal symbionts are related during spring reactivation. To our knowledge, we show for the first time that sugar concentrations in the fine roots are significantly positively correlated with EM-derived enzymatic activities. In the P. sylvestris stand, both C- and N-degrading enzymes showed significant positive correlations with fine root sugar concentrations. In the F. sylvatica stand, sugar concentrations in the fine roots were explicitly correlated with the activity of N-degrading enzymes of the EM fungal symbionts. This may reflect differences in the specific N demand during spring reactivation between both tree species. While the period of spring reactivation exerts a strong N demand in deciduous trees due to the establishment of new leaves, N demand in coniferous trees is rather equally strong throughout the growing season. Therefore, our analysis reinforces the view of a host-dependent regulation of the activity of EM fungi (Nehls et al. 2001). Moreover, EM fungi contribute substantially to the activity of NAG in the soil during spring reactivation. We suggest that fungal necromass is broken down by EM fungi to mobilize N, and potentially C, from chitin (Drigo et al. 2012). Thus, the trees´ resource demand may directly affect the mineralization of SOM.
Abbreviations
- C:
-
Carbon
- CEL:
-
Cellobiohydroloase
- EM:
-
Ectomycorrhizal
- GLU:
-
ß-Glucosidase
- LAP:
-
Leucine-aminopeptidase
- N:
-
Nitrogen
- NAG:
-
N-acetyl-glucosaminidase
- NSC:
-
Non-structural carbohydrates
- P:
-
Phosphorus
- SOM:
-
Soil organic matter
- XYL:
-
ß-Xylosidase
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Acknowledgments
We thank Martin Pollak and Werner Kummer from the Esterházy forest holding for their permission to conduct our research at their forest.
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Open access funding provided by University of Natural Resources and Life Sciences Vienna (BOKU). This work was funded by a Marie Curie Actions Career Integration Grant GPF333996 LINKTOFUN and by the Ministry of Education, Youth and Sports of CR within the National Sustainability Program NPU I, grant No. LO1415
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Rosinger, C., Sandén, H. & Godbold, D.L. Non-structural carbohydrate concentrations of Fagus sylvatica and Pinus sylvestris fine roots are linked to ectomycorrhizal enzymatic activity during spring reactivation. Mycorrhiza 30, 197–210 (2020). https://doi.org/10.1007/s00572-020-00939-x
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DOI: https://doi.org/10.1007/s00572-020-00939-x