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Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.
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SYSNO ASEP 0456197 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production. Tvůrce(i) Pelclová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Kačer, P. (CZ)
Fenclová, Z. (CZ)
Vlčková, Š. (CZ)
Syslová, K. (CZ)
Navrátil, Tomáš (UFCH-W) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Zíková, Naděžda (UCHP-M) RID, ORCID, SAI
Barošová, H. (CZ)
Turci, F. (IT)
Komarc, M. (CZ)
Pelcl, T. (CZ)
Běláček, J. (CZ)
Kukutschová, J. (CZ)
Zakharov, S. (CZ)Zdroj.dok. Journal of Breath Research. - : Institute of Physics Publishing - ISSN 1752-7155
Roč. 10, č. 1 (2016), s. 016004Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova nanoparticles ; exhaled breath condensate ; oxidative stress ; urine ; occupational exposure ; Fe2O3 ; Fe3O4 Vědní obor RIV DN - Vliv životního prostředí na zdraví Obor OECD Public and environmental health Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie Institucionální podpora UCHP-M - RVO:67985858 ; UFCH-W - RVO:61388955 UT WOS 000375749700006 EID SCOPUS 84983215770 DOI 10.1088/1752-7155/10/1/016004 Anotace Markers of oxidative stress and inflammation were analysed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43±7 years) exposed to iron oxide aerosol for an average of 10±4 years and 14 controls (mean age 39±4 years) by liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083mg m(-3) (IQR 0.063-0.133mg m(-3)) and the median particle concentration was 66800 particles cm(-3) (IQR 16900-86900 particles cm(-3)). In addition, more than 80% of particles were smaller than 100nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p<0.001), and C11 (p<0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC.
Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2016
Počet záznamů: 1