Počet záznamů: 1  

Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.

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    SYSNO ASEP0456197
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevOxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.
    Tvůrce(i) Pelclová, D. (CZ)
    Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
    Kačer, P. (CZ)
    Fenclová, Z. (CZ)
    Vlčková, Š. (CZ)
    Syslová, K. (CZ)
    Navrátil, Tomáš (UFCH-W) RID, ORCID
    Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
    Zíková, Naděžda (UCHP-M) RID, ORCID, SAI
    Barošová, H. (CZ)
    Turci, F. (IT)
    Komarc, M. (CZ)
    Pelcl, T. (CZ)
    Běláček, J. (CZ)
    Kukutschová, J. (CZ)
    Zakharov, S. (CZ)
    Zdroj.dok.Journal of Breath Research - ISSN 1752-7155
    Roč. 10, č. 1 (2016), s. 016004
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovananoparticles ; exhaled breath condensate ; oxidative stress ; urine ; occupational exposure ; Fe2O3 ; Fe3O4
    Vědní obor RIVDN - Vliv životního prostředí na zdraví
    Obor OECDPublic and environmental health
    Vědní obor RIV – spolupráceÚstav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie
    Institucionální podporaUCHP-M - RVO:67985858 ; UFCH-W - RVO:61388955
    UT WOS000375749700006
    EID SCOPUS84983215770
    DOI10.1088/1752-7155/10/1/016004
    AnotaceMarkers of oxidative stress and inflammation were analysed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43±7 years) exposed to iron oxide aerosol for an average of 10±4 years and 14 controls (mean age 39±4 years) by liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083mg m(-3) (IQR 0.063-0.133mg m(-3)) and the median particle concentration was 66800 particles cm(-3) (IQR 16900-86900 particles cm(-3)). In addition, more than 80% of particles were smaller than 100nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p<0.001), and C11 (p<0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC.
    PracovištěÚstav chemických procesů
    KontaktEva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227
    Rok sběru2016
Počet záznamů: 1