Počet záznamů: 1

Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

SYSNO ASEP	0558807
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro
Tvůrce(i)	Klimek, K. (PL) Benko, A. (PL) Vandrovcová, Marta (FGU-C)_{RID, ORCID} Trávníčková, Martina (FGU-C)_{RID, ORCID, SAI} Douglas, T. E. L. (GB) Tarczynska, M. (PL) Brož, Antonín (FGU-C)_{RID, ORCID, SAI} Gaweda, K. (PL) Ginalska, G. (PL) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Číslo článku	212724
Zdroj.dok.	Biomaterials Advances. - : Elsevier Roč. 135, April (2022)
Poč.str.	19 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	beta-1,3-Glucan ; biphasic scaffold ; osteochondral defects ; regenerative medicine ; stem cells ; tissue engineering
Obor OECD	Biomaterials (as related to medical implants, devices, sensors)
CEP	NU20-08-00208 GA MZd - Ministerstvo zdravotnictví
	LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000812231700001
EID SCOPUS	85131954631
DOI	10.1016/j.bioadv.2022.212724
Anotace	Osteochondral defects remain a huge problem in medicine today. Biomimetic bi- or multi-phasic scaffolds constitute a very promising alternative to osteochondral autografts and allografts. In this study, a new curdlanbased scaffold was designed for osteochondral tissue engineering applications. To achieve biomimetic properties, it was enriched with a protein component ??? whey protein isolate as well as a ceramic ingredient ??? hydroxyapatite granules. The scaffold was fabricated via a simple and cost-efficient method, which represents a significant advantage. Importantly, this technique allowed generation of a scaffold with two distinct, but integrated phases. Scanning electron microcopy and optical profilometry observations demonstrated that phases of biomaterial possessed different structural properties. The top layer of the biomaterial (mimicking the cartilage) was smoother than the bottom one (mimicking the subchondral bone), which is beneficial from a biological point of view because unlike bone, cartilage is a smooth tissue. Moreover, mechanical testing showed that the top layer of the biomaterial had mechanical properties close to those of natural cartilage. Although the mechanical properties of the bottom layer of scaffold were lower than those of the subchondral bone, it was still higher than in many analogous systems. Most importantly, cell culture experiments indicated that the biomaterial possessed high cytocompatibility towards adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in vitro. Both phases of the scaffold enhanced cell adhesion, proliferation, and chondrogenic differentiation of stem cells (revealing its chondroinductive properties in vitro) as well as osteogenic differentiation of these cells (revealing its osteoinductive properties in vitro). Given all features of the novel curdlan-based scaffold, it is worth noting that it may be considered as promising candidate for osteochondral tissue engineering applications.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2023
Elektronická adresa	https://doi.org/10.1016/j.bioadv.2022.212724

Počet záznamů: 1