Počet záznamů: 1
Metabolomics of Cerebrospinal Fluid in Multiple Sclerosis Compared With Healthy Controls: A Pilot Study
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SYSNO ASEP 0558392 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Metabolomics of Cerebrospinal Fluid in Multiple Sclerosis Compared With Healthy Controls: A Pilot Study Tvůrce(i) Židó, M. (CZ)
Kačer, D. (CZ)
Valeš, Karel (FGU-C) RID, ORCID, SAI
Svobodová, Z. (CZ)
Zimová, D. (CZ)
Štětkářová, I. (CZ)Číslo článku 874121 Zdroj.dok. Frontiers in Neurology. - : Frontiers Media - ISSN 1664-2295
Roč. 13, May 26 (2022)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova multiple sclerosis ; CSF ; metabolomics ; biomarker ; fatty acids ; amino acids ; arginine Obor OECD Neurosciences (including psychophysiology Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000808251100001 EID SCOPUS 85132113090 DOI 10.3389/fneur.2022.874121 Anotace BackgroundMultiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) leading to the loss of myelin and axons. Diagnosis is based on clinical findings, MRI, and analysis of cerebrospinal fluid (CSF). CSF is an ultrafiltrate of plasma and reflects inflammatory processes in the CNS. The aim of this study was to perform metabolomics analysis of CSF in patients after the first attack of MS and healthy controls and try to find new specific analytes for MS including those potentially predicting disease activities at the onset. MethodsWe collected CSF from 19 patients (16 females, aged 19-55 years) after the first attack of clinical symptoms who fulfilled revised McDonald criteria of MS and CSF of 19 controls (16 females, aged 19-50 years). Analyses of CSF samples were provided using the high-performance liquid chromatography system coupled with a mass spectrometer with a high-resolution detector (TripleTOF 5600, AB Sciex, Canada). ResultsApproximately 130 selected analytes were identified, and 30 of them were verified. During the targeted analysis, a significant decrease in arginine and histidine and a less significant decrease in the levels of asparagine, leucine/isoleucine, and tryptophan, together with a significant increase of palmitic acid in the patient group, were found. ConclusionWe observed significant differences in amino and fatty acids in the CSF of newly diagnosed patients with MS in comparison with controls. The most significant changes were observed in levels of arginine, histidine, and palmitic acid that may predict inflammatory disease activity. Further studies are necessary to support these findings as potential biomarkers of MS. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://doi.org/10.3389/fneur.2022.874121
Počet záznamů: 1