Počet záznamů: 1
Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind, randomised clinical trial
- 1.
SYSNO ASEP 0556203 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind, randomised clinical trial Tvůrce(i) Fisk, H. L. (GB)
Childs, C. E. (GB)
Miles, E. A. (GB)
Ayres, R. (GB)
Noakes, P. S. (GB)
Paras-Chavez, C. (GB)
Kuda, Ondřej (FGU-C) RID, ORCID, SAI
Kopecký, Jan (FGU-C) RID, ORCID
Antoun, E. (GB)
Lillycrop, K. A. (GB)
Calder, P. C. (GB)Číslo článku 103909 Zdroj.dok. EBioMedicine. - : Elsevier - ISSN 2352-3964
Roč. 77, Mar (2022)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova adipose tissue ; inflammation ; obesity ; LC n-3 PUFA ; lipids ; immune system Obor OECD Endocrinology and metabolism (including diabetes, hormones) Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000794033100006 EID SCOPUS 85125494272 DOI 10.1016/j.ebiom.2022.103909 Anotace Background:Obesity is associated with enhanced inflammation. However, investigation in human subcutaneous white adipose tissue (scWAT) is limited and the mechanisms by which inflammation occurs have not been well elucidated. Marine long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFAs) have anti-inflammatory actions and may reduce scWAT inflammation.Methods:Subcutaneous white adipose tissue (scWAT) biopsies were collected from individuals living with obesity (n=45) and normal weight individuals (n=39) prior to and following a 12-week intervention with either 3 g/day of a fish oil concentrate (providing 1.1 g eicosapentaenoic acid (EPA) + 0.8 g docosahexaenoic acid (DHA)) or 3 g/day of corn oil. ScWAT fatty acid, oxylipin, and transcriptome profiles were assessed by gas chromatography, ultra-pure liquid chromatography tandem mass spectrometry, RNA sequencing and qRT-PCR, respectively.Findings:Obesity was associated with greater scWAT inflammation demonstrated by lower concentrations of specialised pro-resolving mediators (SPMs) and hydroxy-DHA metabolites and an altered transcriptome with differential expression of genes involved in LC n-3 PUFA activation, oxylipin synthesis, inflammation, and immune response. Intervention with LC n-3 PUFAs increased their respective metabolites including the SPM precursor 14-hydroxy-DHA in normal weight individuals and decreased arachidonic acid derived metabolites and expression of genes involved in immune and inflammatory response with a greater effect in normal weight individuals. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://doi.org/10.1016/j.ebiom.2022.103909
Počet záznamů: 1