Počet záznamů: 1  

The possible role of hypoxia in the affected tissue of relapsed clubfoot

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    SYSNO ASEP0556129
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevThe possible role of hypoxia in the affected tissue of relapsed clubfoot
    Tvůrce(i) Novotný, T. (CZ)
    Eckhardt, Adam (FGU-C) RID, ORCID
    Doubková, Martina (FGU-C) ORCID, RID
    Knitlová, Jarmila (FGU-C) RID, ORCID
    Vondrášek, David (FGU-C) ORCID, SAI
    Vaňásková, E. (CZ)
    Ošťádal, M. (CZ)
    Uhlík, J. (CZ)
    Bačáková, Lucie (FGU-C) RID, ORCID
    Musílková, Jana (FGU-C) RID, ORCID
    Celkový počet autorů10
    Číslo článku4462
    Zdroj.dok.Scientific Reports - ISSN 2045-2322
    Roč. 12, č. 1 (2022)
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovamatrix metalloproteinases ; ponseti method ; hemoglobin ; expression ; beta ; growth ; alpha ; proliferation ; angiogenesis ; hif-1-alpha
    Obor OECDOrthopaedics
    CEPNV17-31564A GA MZd - Ministerstvo zdravotnictví
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000769466200014
    EID SCOPUS85126640231
    DOI10.1038/s41598-022-08519-z
    AnotaceOur aim was to study the expression of hypoxia-related proteins as a possible regulatory pathway in the contracted side tissue of relapsed clubfoot. We compared the expression of hypoxia-related proteins in the tissue of the contracted (medial) side of relapsed clubfoot, and in the tissue of the non-contracted (lateral) side of relapsed clubfoot. Tissue samples from ten patients were analyzed by immunohistochemistry and image analysis, Real-time PCR and Mass Spectrometry to evaluate the differences in protein composition and gene expression. We found a significant increase in the levels of smooth muscle actin, transforming growth factor-beta, hypoxia-inducible factor 1 alpha, lysyl oxidase, lysyl oxidase-like 2, tenascin C, matrix metalloproteinase-2, matrix metalloproteinase-9, fibronectin, collagen types III and VI, hemoglobin subunit alpha and hemoglobin subunit beta, and an overexpression of ACTA2, FN1, TGFB1, HIF1A and MMP2 genes in the contracted medial side tissue of clubfoot. In the affected tissue, we have identified an increase in the level of hypoxia-related proteins, together with an overexpression of corresponding genes. Our results suggest that the hypoxia-associated pathway is potentially a factor contributing to the etiology of clubfoot relapses, as it stimulates both angioproliferation and fibroproliferation, which are considered to be key factors in the progression and development of relapses.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2023
    Elektronická adresahttps://doi.org/10.1038/s41598-022-08519-z
Počet záznamů: 1