Počet záznamů: 1

The possible role of hypoxia in the affected tissue of relapsed clubfoot

SYSNO ASEP	0556129
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The possible role of hypoxia in the affected tissue of relapsed clubfoot
Tvůrce(i)	Novotný, T. (CZ) Eckhardt, Adam (FGU-C)_{RID, ORCID} Doubková, Martina (FGU-C)_{ORCID, RID} Knitlová, Jarmila (FGU-C)_{RID, ORCID} Vondrášek, David (FGU-C)_{ORCID, SAI} Vaňásková, E. (CZ) Ošťádal, M. (CZ) Uhlík, J. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID} Musílková, Jana (FGU-C)_{RID, ORCID}
Celkový počet autorů	10
Číslo článku	4462
Zdroj.dok.	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 12, č. 1 (2022)
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	matrix metalloproteinases ; ponseti method ; hemoglobin ; expression ; beta ; growth ; alpha ; proliferation ; angiogenesis ; hif-1-alpha
Obor OECD	Orthopaedics
CEP	NV17-31564A GA MZd - Ministerstvo zdravotnictví
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000769466200014
EID SCOPUS	85126640231
DOI	10.1038/s41598-022-08519-z
Anotace	Our aim was to study the expression of hypoxia-related proteins as a possible regulatory pathway in the contracted side tissue of relapsed clubfoot. We compared the expression of hypoxia-related proteins in the tissue of the contracted (medial) side of relapsed clubfoot, and in the tissue of the non-contracted (lateral) side of relapsed clubfoot. Tissue samples from ten patients were analyzed by immunohistochemistry and image analysis, Real-time PCR and Mass Spectrometry to evaluate the differences in protein composition and gene expression. We found a significant increase in the levels of smooth muscle actin, transforming growth factor-beta, hypoxia-inducible factor 1 alpha, lysyl oxidase, lysyl oxidase-like 2, tenascin C, matrix metalloproteinase-2, matrix metalloproteinase-9, fibronectin, collagen types III and VI, hemoglobin subunit alpha and hemoglobin subunit beta, and an overexpression of ACTA2, FN1, TGFB1, HIF1A and MMP2 genes in the contracted medial side tissue of clubfoot. In the affected tissue, we have identified an increase in the level of hypoxia-related proteins, together with an overexpression of corresponding genes. Our results suggest that the hypoxia-associated pathway is potentially a factor contributing to the etiology of clubfoot relapses, as it stimulates both angioproliferation and fibroproliferation, which are considered to be key factors in the progression and development of relapses.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2023
Elektronická adresa	https://doi.org/10.1038/s41598-022-08519-z

Počet záznamů: 1