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Changing Temperature Conditions during Somatic Embryo Maturation Result in Pinus pinaster Plants with Altered Response to Heat Stress
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SYSNO ASEP 0554678 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Changing Temperature Conditions during Somatic Embryo Maturation Result in Pinus pinaster Plants with Altered Response to Heat Stress Tvůrce(i) Sales, E. (ES)
Canizares, E. (ES)
Pereira, C. (ES)
Pérez-Oliver, M. A. (ES)
Nebauer, S. (ES)
Pavlović, Iva (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Segura, J. (ES)
Arrillaga, I. (ES)Celkový počet autorů 9 Číslo článku 1318 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 3 (2022)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova tolerance ; anatomy ; thermotolerance ; embryogenesis ; variability ; physiology ; expression ; abies ; somatic embryogenesis ; abiotic stress ; resilience ; photosynthesis ; maritime pine ; leaf anatomy Obor OECD Plant sciences, botany CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA20-22875S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000754629600001 EID SCOPUS 85123890355 DOI 10.3390/ijms23031318 Anotace Under the global warming scenario, obtaining plant material with improved tolerance to abiotic stresses is a challenge for afforestation programs. In this work, maritime pine (Pinus pinaster Aiton) plants were produced from somatic embryos matured at different temperatures (18, 23, or 28 degrees C, named after M18, M23, and M28, respectively) and after 2 years in the greenhouse a heat stress treatment (45 degrees C for 3 h/day for 10 days) was applied. Temperature variation during embryo development resulted in altered phenotypes (leaf histology, proline content, photosynthetic rates, and hormone profile) before and after stress. The thickness of chlorenchyma was initially larger in M28 plants, but was significantly reduced after heat stress, while increased in M18 plants. Irrespective of their origin, when these plants were subjected to a heat treatment, relative water content (RWC) and photosynthetic carbon assimilation rates were not significantly affected, although M18 plants increased net photosynthesis rate after 10 days recovery (tR). M18 plants showed proline contents that increased dramatically (2.4-fold) when subjected to heat stress, while proline contents remained unaffected in M23 and M28 plants. Heat stress significantly increased abscisic acid (ABA) content in the needles of maritime pine plants (1.4-, 3.6- and 1.9-fold in M18, M23, and M28 plants, respectively), while indole-3-acetic acid content only increased in needles from M23 plants. After the heat treatment, the total cytokinin contents of needles decreased significantly, particularly in M18 and M28 plants, although levels of active forms (cytokinin bases) did not change in M18 plants. In conclusion, our results suggest that maturation of maritime pine somatic embryos at lower temperature resulted in plants with better performance when subjected to subsequent high temperature stress, as demonstrated by faster and higher proline increase, lower increases in ABA levels, no reduction in active cytokinin, and a better net photosynthesis rate recovery. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3390/ijms23031318
Počet záznamů: 1