Počet záznamů: 1  

Complex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads

  1. 1.
    SYSNO ASEP0548344
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevComplex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads
    Tvůrce(i) Vondrak, Tihana (BC-A) RID
    Oliveira, Ludmila (BC-A) RID, ORCID
    Novák, Petr (BC-A) RID, ORCID
    Koblížková, Andrea (BC-A)
    Neumann, Pavel (BC-A) RID, ORCID
    Macas, Jiří (BC-A) RID, ORCID, SAI
    Celkový počet autorů6
    Zdroj.dok.Computational and Structural Biotechnology Journal. - : Elsevier - ISSN 2001-0370
    Roč. 19, Apr 22 (2021), s. 2179-2189
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.SE - Švédsko
    Klíč. slovaSatellite DNA ; Heterochromatin ; Oxford Nanopore sequencing ; Fluorescence in situ hybridization ; Holocentric chromosomes ; LINE elements
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDGenetics and heredity (medical genetics to be 3)
    CEPGA20-24252S GA ČR - Grantová agentura ČR
    LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Výzkumná infrastrukturaELIXIR-CZ II - 90131 - Ústav organické chemie a biochemie AV ČR, v. v. i.
    Způsob publikováníOpen access
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000684934600009
    EID SCOPUS85104729200
    DOI10.1016/j.csbj.2021.04.011
    AnotaceRepeat-rich regions of higher plant genomes are usually associated with constitutive heterochromatin, a specific type of chromatin that forms tightly packed nuclear chromocenters and chromosome bands. There is a large body of cytogenetic evidence that these chromosome regions are often composed of tandemly organized satellite DNA. However, comparatively little is known about the sequence arrangement within heterochromatic regions, which are difficult to assemble due to their repeated nature. Here, we explore long-range sequence organization of heterochromatin regions containing the major satellite repeat CUS-TR24 in the holocentric plant Cuscuta europaea. Using a combination of ultra-long read sequencing with assembly-free sequence analysis, we reveal the complex structure of these loci, which are composed of short arrays of CUS-TR24 interrupted frequently by emerging simple sequence repeats and targeted insertions of a specific lineage of LINE retrotransposons. These data suggest that the organization of satellite repeats constituting heterochromatic chromosome bands can be more complex than previously envisioned, and demonstrate that heterochromatin organization can be efficiently investigated without the need for genome assembly. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2022
    Elektronická adresahttps://www.sciencedirect.com/science/article/pii/S2001037021001227?via%3Dihub
Počet záznamů: 1  

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