Počet záznamů: 1  

TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley

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    SYSNO ASEP0547377
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevTALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
    Tvůrce(i) Takáč, T. (CZ)
    Křenek, P. (CZ)
    Komis, G. (CZ)
    Vadovič, P. (CZ)
    Ovečka, M. (CZ)
    Ohnoutková, Ludmila (UEB-Q) RID, ORCID
    Pechan, T. (US)
    Kašpárek, Petr (UMG-J)
    Tichá, T. (CZ)
    Basheer, J. (CZ)
    Arick, M. (US)
    Šamaj, J. (CZ)
    Celkový počet autorů12
    Číslo článku666229
    Zdroj.dok.Frontiers in Plant Science - ISSN 1664-462X
    Roč. 12, APR 29 (2021)
    Poč.str.23 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovabarley ; chitinases ; flagellin ; HvMPK3 ; PR proteins ; proteomics ; root hairs ; talen
    Obor OECDBiochemistry and molecular biology
    CEPEF16_019/0000827 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaUEB-Q - RVO:61389030 ; UMG-J - RVO:68378050
    UT WOS000649834600001
    EID SCOPUS85105936605
    DOI10.3389/fpls.2021.666229
    AnotaceMitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22.
    PracovištěÚstav experimentální botaniky
    KontaktDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Rok sběru2022
    Elektronická adresahttp://doi.org/10.3389/fpls.2021.666229
Počet záznamů: 1