Počet záznamů: 1
Excess ischemic tachyarrhythmias trigger protection infarction in rats
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SYSNO ASEP 0546478 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Excess ischemic tachyarrhythmias trigger protection infarction in rats Tvůrce(i) Neckář, Jan (FGU-C) RID, ORCID
Alánová, Petra (FGU-C) RID, ORCID
Olejníčková, Veronika (FGU-C) RID, ORCID, SAI
Papoušek, František (FGU-C)
Hejnová, L. (CZ)
Šilhavý, Jan (FGU-C) RID, ORCID
Behuliak, Michal (FGU-C) RID, ORCID
Bencze, Michal (FGU-C) RID, ORCID, SAI
Hrdlička, Jaroslav (FGU-C) ORCID, RID
Vecka, M. (CZ)
Jarkovská, D. (CZ)
Švíglerová, J. (CZ)
Mistrová, E. (CZ)
Štengl, M. (CZ)
Novotný, J. (CZ)
Ošťádal, Bohuslav (FGU-C) RID, ORCID
Pravenec, Michal (FGU-C) RID, ORCID
Kolář, František (FGU-C) RID, ORCID, SAIZdroj.dok. Clinical science. - : Portland Press - ISSN 0143-5221
Roč. 135, č. 17 (2021), s. 2143-2163Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova C-reactive protein ; heart ; metabolomics ; myocardial infarction ; remote ischemic perconditioning ; ventricular arrhythmias Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) CEP GA18-03207S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000702536100001 EID SCOPUS 85116954913 DOI 10.1042/CS20210648 Anotace Increased level of C-reactive protein (CRP) is a risk factor for cardiovascular diseases, including myocardial infarction and hypertension. Here, we analyzed the effects of CRP overexpression on cardiac susceptibility to ischemia/reperfusion (I/R) injury in adult spontaneously hypertensive rats (SHR) expressing human CRP transgene (SHR-CRP). Using an in vivo model of coronary artery occlusion, we found that transgenic expression of CRP predisposed SHR-CRP to repeated and prolonged ventricular tachyarrhythmias. Excessive ischemic arrhythmias in SHR-CRP led to a significant reduction in infarct size (IS) compared with SHR. The proarrhythmic phenotype in SHR-CRP was associated with altered heart and plasma eicosanoids, myocardial composition of fatty acids (FAs) in phospholipids, and autonomic nervous system imbalance before ischemia. To explain unexpected IS-limiting effect in SHR-CRP, we performed metabolomic analysis of plasma before and after ischemia. We also determined cardiac ischemic tolerance in hearts subjected to remote ischemic perconditioning (RIPer) and in hearts ex vivo. Acute ischemia in SHR-CRP markedly increased plasma levels of multiple potent cardioprotective molecules that could reduce IS at reperfusion. RIPer provided IS-limiting effect in SHR that was comparable with myocardial infarction observed in naïve SHR-CRP. In hearts ex vivo, IS did not differ between the strains, suggesting that extra-cardiac factors play a crucial role in protection. Our study shows that transgenic expression of human CRP predisposes SHR-CRP to excess ischemic ventricular tachyarrhythmias associated with a drop of pump function that triggers myocardial salvage against lethal I/R injury likely mediated by protective substances released to blood from hypoxic organs and tissue at reperfusion. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1042/CS20210648
Počet záznamů: 1