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Minority potassium-uptake system Trk2 has a crucial role in yeast survival of glucose-induced cell death
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SYSNO ASEP 0545535 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název Minority potassium-uptake system Trk2 has a crucial role in yeast survival of glucose-induced cell death Tvůrce(i) Dušková, Michala (FGU-C) RID, ORCID
Cmunt, Denis (FGU-C) ORCID
Papoušková, Klára (FGU-C) RID, ORCID
Masaryk, Jakub (FGU-C)
Sychrová, Hana (FGU-C) RID, ORCIDČíslo článku 001065 Zdroj.dok. Microbiology. - : Microbiology Society - ISSN 1350-0872
Roč. 167, č. 6 (2021)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova GICD ; potassium uptake ; stationary cells ; Saccharomyces cerevisiae ; thermotolerance ; ATP content Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA20-04420S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 EID SCOPUS 85111784795 DOI 10.1099/mic.0.001065 Anotace The existence of programmed cell death in Saccharomyces cerevisiae has been reported for many years. Glucose induces the death of S. cerevisiae in the absence of additional nutrients within a few hours, and the absence of active potassium uptake makes cells highly sensitive to this process. S. cerevisiae cells possess two transporters, Trk1 and Trk2, which ensure a high intracellular concentration of potassium, necessary for many physiological processes. Trk1 is the major system responsible for potassium acquisition in growing and dividing cells. The contribution of Trk2 to potassium uptake in growing cells is almost negligible, but Trk2 becomes crucial for stationary cells for their survival of some stresses, e.g. anhydrobiosis. As a new finding, we show that both Trk systems contribute to the relative thermotolerance of S. cerevisiae BY4741. Our results also demonstrate that Trk2 is much more important for the cell survival of glucose-induced cell death than Trk1, and that stationary cells deficient in active potassium uptake lose their ATP stocks more rapidly than cells with functional Trk systems. This is probably due to the upregulated activity of plasma-membrane Pma1 H+-ATPase, and consequently, it is the reason why these cells die earlier than cells with functional active potassium uptake. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1099/mic.0.001065
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