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Recommendations on the quantitative analysis of pheophorbides, photosensitizers present in algal biomass intended as food supplement
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SYSNO ASEP 0544796 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Recommendations on the quantitative analysis of pheophorbides, photosensitizers present in algal biomass intended as food supplement Tvůrce(i) Činčárová, Dominika (MBU-M)
Hájek, Jan (MBU-M) ORCID
Dobřichovský, M. (CZ)
Lukeš, Martin (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCIDCelkový počet autorů 5 Číslo článku 102298 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 56, JUNE 2021 (2021)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Pheophorbide a ; Analysis ; Extraction ; Chlorophyllase inactivation ; Algae ; Chlorophyll degradation Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP TN01000048 GA TA ČR - Technologická agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000663410300004 EID SCOPUS 85105254433 DOI 10.1016/j.algal.2021.102298 Anotace Pheophorbide a is the major photosensitizer present in algal biomass and its presence in food supplement was linked to human health issues in the past. Although several pheophorbide a quantification methods have been proposed including the United States Pharmacopeia-National Formulary protocol (USP-NFP), there are open methodological issues which make their results questionable. In the present study we have tested optimal extraction conditions for pheophorbide a from a disintegrated biomass of green algae Chlorella and Haematococcus, widely used as food supplement. Further we have compared the quantification of pheophorbide a in algal extracts using HPLC-HRMS with the spectrophotometric quantification after USP-NFP in Haematococcus biomass and oleoresin samples. We have demonstrated that USP-NFP provide considerably (1.7-2.4 times) higher pheophorbide contents compared to HPLC-HRMS quantification using a pheophorbide a analytical standard. This was mainly caused by the fact that USP-NFP integrates the concentration of all pheophorbide variants present in the samples. We have detected nine pheophorbide analogues in the original algal sample as well as in the final sample of USP-NFP, of which pheophorbide a represented only similar to 40%. However, this explains the values obtained by USP-NFP only partly. We also show that 60% acetone is the most suitable extraction solvent for pheophorbide a from various algal samples, however, we noted that such extracts might exhibit high activity of chlorophyllase generating pheophorbide a during the extraction process. This activity differed between studied Chlorella and Haematococcus biomass extracts. We have achieved the chlorophyllase inactivation by a short-term high-temperature treatment (90 degrees C, 5 min). Acceptable stability of pheophorbide a and only minor degradation of chlorophyll to pheophorbide a has been observed at these conditions. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S221192642100117X?via%3Dihub
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