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Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration
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SYSNO ASEP 0543905 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration Tvůrce(i) Dauss, E. (LT)
Papoušková, Klára (FGU-C) RID, ORCID
Sychrová, Hana (FGU-C) RID, ORCID
Rapoport, A. (LT)Zdroj.dok. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. - : Springer - ISSN 0003-6072
Roč. 114, č. 7 (2021), s. 1069-1077Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova anhydrobiosis ; dehydration-rehydration ; yeast ; endoplasmic reticulum ; endoplasmic reticulum protein Ist2 Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LTC20006 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000639508200002 EID SCOPUS 85104409085 DOI 10.1007/s10482-021-01578-8 Anotace Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2 Delta), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2 increment upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2 increment strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1007/s10482-021-01578-8
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