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Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations
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SYSNO ASEP 0543809 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations Tvůrce(i) Konášová, Renáta (UOCHB-X) ORCID
Butnariu, Maria (UOCHB-X) ORCID
Šolínová, Veronika (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCID
Koval, Dušan (UOCHB-X) RID, ORCIDČíslo článku 338789 Zdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1178, Sep 15 (2021)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova electroosmotic flow ; capillary electrophoresis ; mass spectrometry ; permanent polymeric coating ; sorption suppression Obor OECD Analytical chemistry CEP GA20-03899S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000672564300008 EID SCOPUS 85109129026 DOI 10.1016/j.aca.2021.338789 Anotace Electroosmotic flow (EOF) plays a pivotal role in optimization of capillary electrophoresis (CE) separations of (bio)molecules and (bio)particles. EOF velocity is directly related to analysis time, peak resolution and separation efficiency. Here, we report a concept of charged polymer coatings of the inner fused silica capillary wall, which allows anodic EOF with mobility ranging from 0 to ∼(30–40) × 10−9 m2V−1s−1. The capillary wall is modified by covalently bound cationic copolymer poly(acrylamide-co-(3-acrylamidopropyl)trimethylammonium chloride) (PAMAPTAC) containing variable ratio of the charged monomer in the 0–60 mol. % interval. The EOF mobility showed minor variability with composition of background electrolyte (BGE) and pH in the 2–10 interval. The coatings were evaluated by CE-UV and nanospray CE-MS in the counter-EOF arrangement for a series of basic drug molecules in acetic acid based acidic BGE. Tunable EOF velocity was demonstrated as a useful tool for optimization of peak resolution, separation efficiency and migration time of analytes. Electrostatic repulsion of positively charged capillary surface was shown as beneficial for suppression of analyte adsorption, notably for hydrophobic cationic analytes. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2022 Elektronická adresa https://doi.org/10.1016/j.aca.2021.338789
Počet záznamů: 1