Počet záznamů: 1
Biocompatible polypeptide nanogel: effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation
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SYSNO ASEP 0542700 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biocompatible polypeptide nanogel: effect of surfactants on nanogelation in inverse miniemulsion, in vivo biodistribution and blood clearance evaluation Tvůrce(i) Oleshchuk, Diana (UMCH-V) ORCID
Šálek, Petr (UMCH-V) RID, ORCID
Dvořáková, Jana (UMCH-V) RID, ORCID
Kučka, Jan (UMCH-V) RID, ORCID
Pavlova, Ewa (UMCH-V) RID
Francová, P. (CZ)
Šefc, L. (CZ)
Proks, Vladimír (UMCH-V) RID, ORCIDČíslo článku 111865 Zdroj.dok. Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931
Roč. 126, July (2021)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova blood clearance ; inverse miniemulsion ; in vivo biodistribution Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) CEP GA18-03224S GA ČR - Grantová agentura ČR GA18-07983S GA ČR - Grantová agentura ČR Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000663454800004 EID SCOPUS 85106371851 DOI 10.1016/j.msec.2021.111865 Anotace Horseradish peroxidase (HRP)/H2O2-mediated crosslinking of polypeptides in inverse miniemulsion is a promising approach for the development of next-generation biocompatible and biodegradable nanogels. Herein, we present a fundamental investigation of the effects of three surfactants and their different concentrations on the (HRP)/H2O2-mediated nanogelation of poly[N5-(2-hydroxyethyl)-l-glutamine-ran-N5-propargyl-l-glutamine-ran-N5-(6-aminohexyl)-l-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-l-glutamine] (PHEG-Tyr) in inverse miniemulsion. The surfactants sorbitan monooleate (SPAN 80), polyoxyethylenesorbitan trioleate (TWEEN 85), and dioctyl sulfosuccinate sodium salt (AOT) were selected and their influence on the nanogel size, size distribution, and morphology was evaluated. The most effective nanogelation stabilization was achieved with 20 wt% nonionic surfactant SPAN 80. The diameter of the hydrogel nanoparticles was 230 nm (dynamic light scattering, DLS) and was confirmed also by nanoparticle tracking analysis (NTA) which showed the diameters ranging from 200 to 300 nm. Microscopy and image analyses showed that the nanogel in the dry state was spherical in shape and had number-average diameter Dn = 26 nm and dispersity Ð = 1.91. In the frozen-hydrated state, the nanogel appeared porous and was larger in size with Dn = 182 nm and Ð = 1.52. Our results indicated that the nanogelation of the polymer precursor required a higher concentration of surfactant than classical inverse miniemulsion polymerization to ensure effective stabilization. The developed polypeptide nanogel was radiolabeled with 125I, and in vivo biodistribution and blood clearance evaluations were performed. We found that the 125I-labeled nanogel was well-biodistributed in the bloodstream, cleared from mouse blood during 48 h by renal and hepatic pathways and did not provoke any sign of toxic effects. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0928493121000035?via%3Dihub
Počet záznamů: 1