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K+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells

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    SYSNO ASEP0542676
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevK+-specific importers Trk1 and Trk2 play different roles in Ca2+ homeostasis and signalling in Saccharomyces cerevisiae cells
    Tvůrce(i) Zimmermannová, Olga (FGU-C) RID, ORCID
    Felcmanová, Kristina (FGU-C)
    Sácká, Lenka (FGU-C)
    Colinet, A. S. (BE)
    Morsomme, P. (BE)
    Sychrová, Hana (FGU-C) RID, ORCID
    Číslo článkufoab015
    Zdroj.dok.FEMS Yeast Research - ISSN 1567-1356
    Roč. 21, č. 3 (2021)
    Poč.str.12 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovapotassium ; calcium ; K+-transporter ; osmotic shock ; Trk1 ; Trk2 ; yeast
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGA20-04420S GA ČR - Grantová agentura ČR
    Způsob publikováníOmezený přístup
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000654733000002
    EID SCOPUS85104047228
    DOI10.1093/femsyr/foab015
    AnotaceThe maintenance of K+ and Ca2+ homeostasis is crucial for many cellular functions. Potassium is accumulated in cells at high concentrations, while the cytosolic level of calcium, to ensure its signalling function, is kept at low levels and transiently increases in response to stresses. We examined Ca2+ homeostasis and Ca2+ signalling in Saccharomyces cerevisiae strains lacking plasma-membrane K+ influx (Trk1 and Trk2) or efflux (Tok1, Nha1 and Ena1-5) systems. The lack of K+ exporters slightly increased the cytosolic Ca2+, but did not alter the Ca2+ tolerance or Ca2+-stress response. In contrast, the K+-importers Trk1 and Trk2 play important and distinct roles in the maintenance of Ca2+ homeostasis. The presence of Trk1 was vital mainly for the growth of cells in the presence of high extracellular Ca2+, whilst the lack of Trk2 doubled steady-state intracellular Ca2+ levels. The absence of both K+ importers highly increased the Ca2+ response to osmotic or CaCl2 stresses and altered the balance between Ca2+ flux from external media and intracellular compartments. In addition, we found Trk2 to be important for the tolerance to high KCl and hygromycin B in cells growing on minimal media. All the data describe new interconnections between potassium and calcium homeostasis in S. cerevisiae.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2022
    Elektronická adresahttps://doi.org/10.1093/femsyr/foab015
Počet záznamů: 1