Počet záznamů: 1  

The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine

  1. 1.
    SYSNO ASEP0542413
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevThe Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine
    Tvůrce(i) Ergang, Peter (FGU-C) RID
    Vagnerová, Karla (FGU-C) RID, ORCID
    Hermanová, Petra (MBU-M) ORCID
    Vodička, Martin (FGU-C) RID, ORCID, SAI
    Jágr, M. (CZ)
    Šrůtková, Dagmar (MBU-M) ORCID
    Dvořáček, V. (CZ)
    Hudcovic, Tomáš (MBU-M) RID, ORCID
    Pácha, Jiří (FGU-C) RID, ORCID
    Číslo článku4229
    Zdroj.dok.International Journal of Molecular Sciences. - : MDPI
    Roč. 22, č. 8 (2021)
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaglucocorticoids ; extra-adrenal steroidogenesis ; 11beta-hydroxysteroid dehydrogenase ; intestine ; microbiome ; anti-CD3 antibody
    Vědní obor RIVED - Fyziologie
    Obor OECDPhysiology (including cytology)
    Vědní obor RIV – spolupráceMikrobiologický ústav - Biochemie
    CEPGA18-02993S GA ČR - Grantová agentura ČR
    GA21-10845S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823 ; MBU-M - RVO:61388971
    UT WOS000644332500001
    EID SCOPUS85104404619
    DOI10.3390/ijms22084229
    AnotaceGlucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1 beta, IL6 and Tnf alpha and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2022
    Elektronická adresahttps://www.mdpi.com/1422-0067/22/8/4229
Počet záznamů: 1