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The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine
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SYSNO ASEP 0542413 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine Tvůrce(i) Ergang, Peter (FGU-C) RID, ORCID
Vagnerová, Karla (FGU-C) RID, ORCID
Hermanová, Petra (MBU-M) ORCID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Jágr, M. (CZ)
Šrůtková, Dagmar (MBU-M) ORCID, RID
Dvořáček, V. (CZ)
Hudcovic, Tomáš (MBU-M) RID, ORCID
Pácha, Jiří (FGU-C) RID, ORCIDČíslo článku 4229 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 8 (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova glucocorticoids ; extra-adrenal steroidogenesis ; 11beta-hydroxysteroid dehydrogenase ; intestine ; microbiome ; anti-CD3 antibody Vědní obor RIV ED - Fyziologie Obor OECD Physiology (including cytology) Vědní obor RIV – spolupráce Mikrobiologický ústav - Biochemie CEP GA18-02993S GA ČR - Grantová agentura ČR GA21-10845S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000644332500001 EID SCOPUS 85104404619 DOI 10.3390/ijms22084229 Anotace Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1 beta, IL6 and Tnf alpha and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/8/4229
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