Počet záznamů: 1

Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies

SYSNO ASEP	0542103
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Impact of three-month morphine withdrawal on rat brain cortex, hippocampus, striatum and cerebellum: proteomic and phosphoproteomic studies
Tvůrce(i)	Ujčíková, Hana (FGU-C)_{RID, ORCID} Hejnová, L. (CZ) Eckhardt, Adam (FGU-C)_{RID, ORCID} Roubalová, Lenka (FGU-C)_{RID, ORCID, SAI} Novotný, J. (CZ) Svoboda, Petr (FGU-C)_{RID, ORCID}
Číslo článku	104975
Zdroj.dok.	Neurochemistry International. - : Elsevier - ISSN 0197-0186 Roč. 144, Mar (2021)
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	long-term morphine withdrawal ; phosphoproteomic analysis ; nLC-MS ; label-free quantification ; 14-3-3 proteins
Vědní obor RIV	ED - Fyziologie
Obor OECD	Physiology (including cytology)
CEP	GA19-03295S GA ČR - Grantová agentura ČR
Způsob publikování	Omezený přístup
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000636076900001
EID SCOPUS	85100027342
DOI	10.1016/j.neuint.2021.104975
Anotace	Opioid addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations. However, there is a lack of information about the changes at both the cellular and molecular levels occurring after cessation of drug administration. The aim of our study was to determine alterations of both phosphoproteome and proteome in selected brain regions of the rats (brain cortex, hippocampus, striatum, and cerebellum) 3 months after cessation of 10-day morphine treatment. Phosphoproteome profiling was performed by Pro-Q® Diamond staining. The gel-based proteomic approach accompanied by label-free quantification (MaxLFQ) was used for characterization of proteome changes.The phosphoproteomic analysis revealed the largest change in the hippocampus (14), only few altered proteins were detected in the forebrain cortex (5), striatum (4), and cerebellum (3). The change of total protein composition, determined by 2D electrophoresis followed by LFQ analysis, identified 22 proteins with significantly altered expression levels in the forebrain cortex, 19 proteins in the hippocampus, 12 in the striatum and 10 in the cerebellum. The majority of altered proteins were functionally related to energy metabolism and cytoskeleton reorganization. As the most important change we regard down-regulation of 14-3-3 proteins in rat cortex and hippocampus.Our findings indicate that i) different parts of the brain respond in a distinct manner to the protracted morphine withdrawal, ii) characterize changes of protein composition in these brain parts, and iii) enlarge the scope of evidence for adaptability and distinct neuroplasticity proceeding in the brain of drug-addicted organism.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://doi.org/10.1016/j.neuint.2021.104975

Počet záznamů: 1