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WALTER: an easy way to online evaluate telomere lengths from terminal restriction fragment analysis
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SYSNO ASEP 0542027 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název WALTER: an easy way to online evaluate telomere lengths from terminal restriction fragment analysis Tvůrce(i) Lycka, M. (CZ)
Peška, Vratislav (BFU-R) RID, ORCID
Demko, M. (CZ)
Spyroglou, I. (CZ)
Kilar, A. (CZ)
Fajkus, Jiří (BFU-R) RID, ORCID
Fojtová, M. (CZ)Celkový počet autorů 7 Číslo článku 145 Zdroj.dok. BMC Bioinformatics. - : BioMed Central - ISSN 1471-2105
Roč. 22, č. 1 (2021)Poč.str. 14 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Telomere length ; Terminal restriction fragments ; Online toolset Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-07027S GA ČR - Grantová agentura ČR GX20-01331X GA ČR - Grantová agentura ČR EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000634776700003 EID SCOPUS 85102696153 DOI 10.1186/s12859-021-04064-0 Anotace BackgroundTelomeres, nucleoprotein structures comprising short tandem repeats and delimiting the ends of linear eukaryotic chromosomes, play an important role in the maintenance of genome stability. Therefore, the determination of the length of telomeres is of high importance for many studies. Over the last years, new methods for the analysis of the length of telomeres have been developed, including those based on PCR or analysis of NGS data. Despite that, terminal restriction fragment (TRF) method remains the gold standard to this day. However, this method lacks universally accepted and precise tool capable to analyse and statistically evaluate TRF results.ResultsTo standardize the processing of TRF results, we have developed WALTER, an online toolset allowing rapid, reproducible, and user-friendly analysis including statistical evaluation of the data. Given its web-based nature, it provides an easily accessible way to analyse TRF data without any need to install additional software.ConclusionsWALTER represents a major upgrade from currently available tools for the image processing of TRF scans. This toolset enables a rapid, highly reproducible, and user-friendly evaluation of almost any TRF scan including in-house statistical evaluation of the data. WALTER platform together with user manual describing the evaluation of TRF scans in detail and presenting tips and troubleshooting, as well as test data to demo the software are available at https://www.ceitec.eu/chromatin-molecular-complexes-jiri-fajkus/rg51/tab?tabId=125#WALTER and the source code at https://github.com/mlyc93/WALTER. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-021-04064-0
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