Počet záznamů: 1

Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle

SYSNO ASEP	0541702
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Gap Junctional Communication via Connexin43 between Purkinje Fibers and Working Myocytes Explains the Epicardial Activation Pattern in the Postnatal Mouse Left Ventricle
Tvůrce(i)	Olejníčková, Veronika (FGU-C)_{RID, ORCID, SAI} Kočka, M. (CZ) Kvasilová, A. (CZ) Kolesová, Hana (FGU-C)_{RID, ORCID, SAI} Dziacky, A. (CZ) Gidor, T. (CZ) Gidor, L. (CZ) Šaňková, B. (CZ) Gregorovičová, Martina (FGU-C)_{RID, ORCID, SAI} Gourdie, R. G. (US) Sedmera, David (FGU-C)_{RID, ORCID, SAI}
Číslo článku	2475
Zdroj.dok.	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 5 (2021)
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	connexin ; cardiac conduction system ; optical mapping ; myocardium ; immunohistochemistry
Vědní obor RIV	ED - Fyziologie
Obor OECD	Physiology (including cytology)
CEP	GA18-03461S GA ČR - Grantová agentura ČR
	LTC17023 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000628306200001
EID SCOPUS	85101697987
DOI	10.3390/ijms22052475
Anotace	The mammalian ventricular myocardium forms a functional syncytium due to flow of electrical current mediated in part by gap junctions localized within intercalated disks. The connexin (Cx) subunit of gap junctions have direct and indirect roles in conduction of electrical impulse from the cardiac pacemaker via the cardiac conduction system (CCS) to working myocytes. Cx43 is the dominant isoform in these channels. We have studied the distribution of Cx43 junctions between the CCS and working myocytes in a transgenic mouse model, which had the His-Purkinje portion of the CCS labeled with green fluorescence protein. The highest number of such connections was found in a region about one-third of ventricular length above the apex, and it correlated with the peak proportion of Purkinje fibers (PFs) to the ventricular myocardium. At this location, on the septal surface of the left ventricle, the insulated left bundle branch split into the uninsulated network of PFs that continued to the free wall anteriorly and posteriorly. The second peak of PF abundance was present in the ventricular apex. Epicardial activation maps correspondingly placed the site of the first activation in the apical region, while some hearts presented more highly located breakthrough sites. Taken together, these results increase our understanding of the physiological pattern of ventricular activation and its morphological underpinning through detailed CCS anatomy and distribution of its gap junctional coupling to the working myocardium.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/1422-0067/22/5/2475

Počet záznamů: 1