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Glucocorticoids reset circadian clock in choroid plexus via period genes

SYSNO ASEP	0541621
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Glucocorticoids reset circadian clock in choroid plexus via period genes
Tvůrce(i)	Liška, Karolína (FGU-C)_ORCID Sládek, Martin (FGU-C)_{RID, ORCID, SAI} Čečmanová, Vendula (FGU-C) Sumová, Alena (FGU-C)_{RID, ORCID}
Zdroj.dok.	Journal of Endocrinology. - : BioScientifica - ISSN 0022-0795 Roč. 248, č. 2 (2021), s. 155-166
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	choroid plexus ; circadian clock ; glucocorticoids ; dexamethasone ; circadian entrainment ; phase-response curve
Vědní obor RIV	ED - Fyziologie
Obor OECD	Physiology (including cytology)
CEP	LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GBP304/12/G069 GA ČR - Grantová agentura ČR
Způsob publikování	Open access s časovým embargem (01.02.2022)
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000629252300010
EID SCOPUS	85102602029
DOI	10.1530/JOE-20-0526
Anotace	The epithelial cells of choroid plexus (CP) in brain ventricles produce cerebrospinal fluid and act as the blood-cerebrospinal fluid barrier. In this study, we confirmed that CP in the 4th ventricle is composed of cellular oscillators that all harbor glucocorticoid receptors and are mutually synchronized to produce a robust clock gene expression rhythm detectable at the tissue level in vivo and in vitro. Animals lacking glucocorticoids (GCs) due to surgical removal of adrenal glands had Per1, Per2, Nr1d1 and Bmal1 clock gene rhythmicity in their CP significantly dampened, whereas subjecting them to daily bouts of synthetic GC analog, dexamethasone (DEX), reinforced those rhythms. We verified these in vivo effects using an in vitro model of organotypic CP explants, depending on the time of its application, DEX significantly increased the amplitude and efficiently reset the phase of the CP clock. The results are the first description of a PRC for a non-neuronal clock in the brain, demonstrating that CP clock shares some properties with the non-neuronal clocks elsewhere in the body. Finally, we found that DEX exhibited multiple synergic effects on the CP clock, including acute activation of Per1 expression and change of PER2 protein turnover rate. The DEX-induced shifts of the CP clock were partially mediated via PKA-ERK1/2 pathway. The results provide the first evidence that the GC rhythm strengthens and entrains the clock in the CP helping thus fine-tune the brain environment according to time of day.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://doi.org/10.1530/JOE-20-0526

Počet záznamů: 1