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Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars

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    SYSNO ASEP0541299
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevComprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
    Tvůrce(i) Lakhneko, O. (SK)
    Danchenko, M. (SK)
    Morgun, B. (UA)
    Kovac, A. (SK)
    Majerova, P. (SK)
    Škultéty, L'udovít (MBU-M) ORCID
    Číslo článku3445
    Zdroj.dok.International Journal of Molecular Sciences. - : MDPI
    Roč. 21, č. 10 (2020)
    Poč.str.21 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaamylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000539312100041
    EID SCOPUS85084786514
    DOI10.3390/ijms21103445
    AnotaceBread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2021
    Elektronická adresahttps://www.mdpi.com/1422-0067/21/10/3445
Počet záznamů: 1