Počet záznamů: 1

Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars

SYSNO ASEP	0541299
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars
Tvůrce(i)	Lakhneko, O. (SK) Danchenko, M. (SK) Morgun, B. (UA) Kovac, A. (SK) Majerova, P. (SK) Škultéty, L'udovít (MBU-M)_{ORCID, RID}
Číslo článku	3445
Zdroj.dok.	International Journal of Molecular Sciences. - : MDPI Roč. 21, č. 10 (2020)
Poč.str.	21 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	amylase-trypsin-inhibitors ; triticum-aestivum l. ; proteomic analysis ; celiac-disease ; Triticum aestivum L ; food quality ; cereal allergens ; discovery proteomics ; gluten ; celiac disease
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
Způsob publikování	Open access
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000539312100041
EID SCOPUS	85084786514
DOI	10.3390/ijms21103445
Anotace	Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins), secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2021
Elektronická adresa	https://www.mdpi.com/1422-0067/21/10/3445

Počet záznamů: 1