Počet záznamů: 1

Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model

SYSNO ASEP	0537796
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cyanobacterial lipopeptides puwainaphycins and minutissamides induce disruptive and pro-inflammatory processes in Caco-2 human intestinal barrier model
Tvůrce(i)	Vašíček, Ondřej (BFU-R)_{ORCID, RID} Hájek, Jan (MBU-M)_ORCID Blahová, L. (CZ) Hrouzek, Pavel (MBU-M)_ORCID Babica, Pavel (BU-J)_{RID, ORCID} Kubala, Lukáš (BFU-R)_{RID, ORCID} Šindlerová, Lenka (BFU-R)_{RID, ORCID}
Celkový počet autorů	7
Číslo článku	101849
Zdroj.dok.	Harmful Algae. - : Elsevier - ISSN 1568-9883 Roč. 96, Jun 2020 (2020)
Poč.str.	8 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	cell ; peptides ; proliferation ; stabilization ; toxicity ; claudins ; health
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
Vědní obor RIV – spolupráce	Mikrobiologický ústav - Hydrologie a limnologie Botanický ústav - Mikrobiologie, virologie
CEP	GA16-24949S GA ČR - Grantová agentura ČR
	LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	BFU-R - RVO:68081707 ; MBU-M - RVO:61388971 ; BU-J - RVO:67985939
UT WOS	000541912700014
EID SCOPUS	85086601564
DOI	10.1016/j.hal.2020.101849
Anotace	Puwainaphycins (PUW) and minutissamides (MIN) are cyanobacterial lipopeptides found in various cyanobacterial species. The first possible target of human exposure to them is intestinal epithelium but effect of PUW/ MIN on enterocytes is not known at all. Using differentiated Caco-2 cells, PUW F was found to be cytotoxic from 5 mu M concentration based on lactate dehydrogenase release assay and total protein concentration. However, it is also able to induce production of interleukin 8 in non-cytotoxic concentrations 1 and 2.5 mu M detected by ELISA. Effects of MIN A and C were similar but less pronounced compared to PUW F. On the other hand, MIN D was the least toxic compound with no significant pro-inflammatory effects. Surprisingly, pro-inflammatory activation of the cells by PUW F and MIN C resulted in an increase in tight junction (TJ) protein claudin 4 expression determined by western blot analysis and confirmed by confocal microscopy. Furthermore, decrease in expression of zonula occludens 3, another TJ protein, was observed after the exposure to PUW F. Taken together, these cytotoxic lipopeptides, especially PUW F, are to be studied more deeply due to their capability to activate and/or deregulate human enterocytes in low concentrations.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2021
Elektronická adresa	https://doi.org/10.1016/j.hal.2020.101849

Počet záznamů: 1