Počet záznamů: 1  

Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease

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    SYSNO ASEP0536067
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevLipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease
    Tvůrce(i) Tomášová, Petra (MBU-M)
    Čermáková, Martina (MBU-M) ORCID
    Pelantová, Helena (MBU-M)
    Vecka, M. (CZ)
    Kratochvílová, H. (CZ)
    Lipš, M. (CZ)
    Lindner, J. (CZ)
    Ivák, P. (CZ)
    Netuka, I. (CZ)
    Šedivá, Blanka (MBU-M)
    Haluzník, M. (CZ)
    Kuzma, Marek (MBU-M) ORCID
    Zdroj.dok.Journal of Proteome Research - ISSN 1535-3893
    Roč. 19, č. 10 (2020), s. 3993-4003
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaepicardial adipose tissue ; subcutaneous adipose tissue ; coronary artery disease
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPLO1509 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000577157000011
    EID SCOPUS85092681739
    DOI10.1021/acs.jproteome.0c00269
    AnotaceCoronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We performed an MS-based lipidomic analysis of subcutaneous and epicardial adipose tissue in 23 patients with coronary artery disease stratified for the presence/absence of type 2 diabetes mellitus and a control group of 13 subjects aiming at identification of factors from epicardial fat contributing to the development of coronary artery disease. The samples of adipose tissues were obtained during elective cardiac surgery. They were extracted and analyzed with and without previous triacylglycerols separation by high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Multi-variate and univariate analyses were performed. Lipidomics data were correlated with biochemical parameters. We identified multiple changes in monoacylglycerols, diacylglycerols, triacylglycerols, glycerophosphatidylserines, glycerophosphatidylethanolamines, glycerophosphatidylcholines, ceramides, sphingomyelins, and derivatives of cholesterol. Observed changes included molecules with fatty acids with odd (15:0, 15:1, 17:0, 17:1) and even (10:0, 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:4, 20:1, 22:0) fatty acids in both types of adipose tissue. More pronounced changes were detected in epicardial adipose tissue compared to subcutaneous adipose tissue of patients with coronary artery disease and type 2 diabetes. Lipidomic analysis of subcutaneous and epicardial adipose tissue revealed different profiles for patients with coronary artery disease and type 2 diabetes, which might be related to coronary artery disease and the presence of type 2 diabetes.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2021
    Elektronická adresahttps://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00269
Počet záznamů: 1