Počet záznamů: 1
Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach
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SYSNO ASEP 0535755 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Markers of acute toxicity of DDT exposure in pancreatic beta-cells determined by a proteomic approach Tvůrce(i) Pavlíková, N. (CZ)
Šrámek, J. (CZ)
Jelínek, M. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Kovář, J. (CZ)Číslo článku e0229430 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 15, č. 10 (2020)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova persistent organic pollutants ; organochlorine pesticides ; er stress Obor OECD Biochemistry and molecular biology CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000588370000016 EID SCOPUS 85094863054 DOI 10.1371/journal.pone.0229430 Anotace Many compounds have the potential to harm pancreatic beta-cells, organochlorine pollutants belong to those compounds. In this work, we aimed to find markers of acute toxicity of p,p'-DDT exposure among proteins expressed in NES2Y human pancreatic beta-cells employing 2-D electrophoresis. We exposed NES2Y cells to a high concentration (150 mu M, LC96 after 72 hours) of p,p'-DDT for 24 and 30 hours and determined proteins with changed expression using 2-D electrophoresis. We have found 22 proteins that changed their expression. They included proteins involved in ER stress (GRP78, and endoplasmin), mitochondrial proteins (GRP75, ECHM, IDH3A, NDUS1, and NDUS3), proteins involved in the maintenance of the cell morphology (EFHD2, TCPA, NDRG1, and ezrin), and some other proteins (HNRPF, HNRH1, K2C8, vimentin, PBDC1, EF2, PCNA, biliverdin reductase, G3BP1, FRIL, and HSP27). The proteins we have identified may serve as indicators of p,p'-DDT toxicity in beta-cells in future studies, including long-term exposure to environmentally relevant concentrations. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229430
Počet záznamů: 1