Počet záznamů: 1
Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
- 1.
SYSNO ASEP 0534790 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11. Tvůrce(i) Sushytskyi, L. (CZ)
Lukáč, Pavol (MBU-M) ORCID, RID
Bleha, R. (CZ)
Čopíková, J. (CZ)
Vannucci, Luca (MBU-M) RID, ORCID
Sinica, A. (CZ)
Kaštánek, P. (CZ)Zdroj.dok. Proceedings of the 15th International Conference on Polysaccharides-Glycoscience. - Praha00 : ChemPubSoc Europe, 2019 / xxxxx x. - ISBN 978-80-88307-04-4 Rozsah stran s. 158-163 Poč.str. 6 s. Forma vydání Tištěná - P Akce The 15th International Conference on Polysaccharides-Glycoscience. Datum konání 13.11.2019 - 15.11.2019 Místo konání Praha Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Mutant alga ; Chlorella vulgaris G11 ; chromatography ; polymer ; cell cultures Vědní obor RIV EC - Imunologie Obor OECD Immunology CEP FV10155 GA MPO - Ministerstvo průmyslu a obchodu Institucionální podpora MBU-M - RVO:61388971 Anotace Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW (>1000 kDa and 98,3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column, and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnans, the latter one as branched xylogalactofuranan of unusual structure. CPS1 shown properties of unstable polimer: according to GPC analysis and by using light scattering and RI detectors it was found, that some fraction of polymers (260-800 kDa) have self-assembling properties in aqueous solution, and MW of this fraction decreased during some time while polimer with initially bigger MW (>1000 kDa) became bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98,3 kDa. Cell toxicity of the products were tested in vitro on cell cultures for different concentrations Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021
Počet záznamů: 1