Počet záznamů: 1

Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein

SYSNO ASEP	0524528
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein
Tvůrce(i)	Šebest, Peter (BFU-R)_ORCID Fojt, Lukáš (BFU-R)_{RID, ORCID} Ostatná, Veronika (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID} Daňhel, Aleš (BFU-R)_{RID, ORCID}
Celkový počet autorů	5
Číslo článku	107436
Zdroj.dok.	Bioelectrochemistry. - : Elsevier - ISSN 1567-5394 Roč. 132, APR 2020 (2020)
Poč.str.	9 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	voltammetric determination ; label ; electrochemistry ; oligonucleotides ; nanoparticles
Vědní obor RIV	CE - Biochemie
Obor OECD	Biochemistry and molecular biology
CEP	EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GJ17-23634Y GA ČR - Grantová agentura ČR
Způsob publikování	Omezený přístup
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000518670600036
EID SCOPUS	85076459677
DOI	10.1016/j.bioelechem.2019.107436
Anotace	Catalytic properties and high adsorption affinity of nucleic acids and proteins to silver amalgam electrode surface make this kind of electrified interface perspective for bioanalytical and biomedical applications. For the first time, a basal-plane pyrolytic graphite electrode (bPGE) has been used as a substrate for electrodeposition of silver amalgam particles (AgAPs). Optimization of the resulting composition, surface morphology and electrochemical properties of the AgAPs was done by scanning electron microscopy with energy disperse X-ray spectroscopy, image processing software and voltammetric detection of electrochemically reducible model organic nitro compound, 4-nitrophenol. Spectro-electrochemical applicability of bPGE-AgAP has been demonstrated by electrolysis of 4-nitrophenol. Simultaneous UV-Vis-chronoamperometry provided information on the number of exchange electrons and the reduction rate constants. Preferential adsorption of the fluorescently labelled calf thymus DNA and the green fluorescent protein (GFP) on the surface of AgAPs was observed by fluorescence microscopy. In contrast to previously studied indium-tin oxide and vapour-deposited gold decorated by AgAPs, herein the presented bPGE-AgAP has provided sufficiently wide negative potential window allowing direct electroanalysis of non labelled DNA and GFP using intrinsic electrochemical signals independently of the fluorescent labelling. The bPGE-AgAP can thus be expected to find application opportunities in protein electrochemistry, (bio) sensor development or in-situ spectro-electrochemical studies. (C) 2019 Elsevier B.V. All rights reserved.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2021
Elektronická adresa	https://www.sciencedirect.com/science/article/pii/S1567539419304098?via%3Dihub

Počet záznamů: 1