Počet záznamů: 1
Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles
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SYSNO ASEP 0524235 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles Tvůrce(i) Šebest, Peter (BFU-R) ORCID
Ostatná, Veronika (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCID
Daňhel, Aleš (BFU-R) RID, ORCIDCelkový počet autorů 4 Číslo článku 113854 Zdroj.dok. Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 859, FEB 15 2020 (2020)Poč.str. 5 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova protein ; voltammetry ; Bovine serum albumin Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GJ17-23634Y GA ČR - Grantová agentura ČR EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000521509300011 EID SCOPUS 85078030466 DOI https://doi.org/10.1016/j.jelechem.2020.113854 Anotace Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S1572665720300370
Počet záznamů: 1