Počet záznamů: 1

Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

SYSNO ASEP	0524235
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles
Tvůrce(i)	Šebest, Peter (BFU-R)_ORCID Ostatná, Veronika (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID} Daňhel, Aleš (BFU-R)_{RID, ORCID}
Celkový počet autorů	4
Číslo článku	113854
Zdroj.dok.	Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657 Roč. 859, FEB 15 2020 (2020)
Poč.str.	5 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	protein ; voltammetry ; Bovine serum albumin
Vědní obor RIV	CG - Elektrochemie
Obor OECD	Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
CEP	GJ17-23634Y GA ČR - Grantová agentura ČR
	EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000521509300011
EID SCOPUS	85078030466
DOI	10.1016/j.jelechem.2020.113854
Anotace	Highly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2021
Elektronická adresa	https://www.sciencedirect.com/science/article/abs/pii/S1572665720300370

Počet záznamů: 1