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Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles

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    SYSNO ASEP0524235
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevConstant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles
    Tvůrce(i) Šebest, Peter (BFU-R) ORCID
    Ostatná, Veronika (BFU-R) RID, ORCID
    Fojta, Miroslav (BFU-R) RID, ORCID
    Daňhel, Aleš (BFU-R) RID, ORCID
    Celkový počet autorů4
    Číslo článku113854
    Zdroj.dok.Journal of Electroanalytical Chemistry - ISSN 1572-6657
    Roč. 859, FEB 15 2020 (2020)
    Poč.str.5 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaprotein ; voltammetry ; Bovine serum albumin
    Vědní obor RIVCG - Elektrochemie
    Obor OECDElectrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    CEPGJ17-23634Y GA ČR - Grantová agentura ČR
    EF15_003/0000477 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaBFU-R - RVO:68081707
    UT WOS000521509300011
    EID SCOPUS85078030466
    DOI10.1016/j.jelechem.2020.113854
    AnotaceHighly sensitive peak H registered by constant-current chronopotentiometric stripping (CPS) analysis relates due to the catalytic hydrogen evolution reaction of proteins adsorbed on mercury-based surfaces including recently introduced silver amalgam particles (AgAPs). Since the peak H allows a structure-sensitive analysis of proteins, including those important in biomedicine and cancer research, it has been systematically studied by CPS analysis using AgAPs in this work. Conditions of AgAPs' electrodeposition on basal plane pyrolytic graphite electrode (bPGE) were optimized towards CPS analysis of model protein (bovine serum albumin, BSA) using peak H. Optimized condition for bPGE-AgAP preparation allowed sensitive CPS detection of either native or denatured BSA down to 10 nM concentration, a discrimination between both forms, and registration of the BSA thermal denaturation kinetic. Obtained results have confirmed the applicability of AgAPs in protein electroanalysis and may contribute to the further development of novel (bio)sensors or (bio)electrochemical applications using micro-volume batch, flow-through or lab-on-chip detection systems. (C) 2020 Elsevier B.V. All rights reserved.
    PracovištěBiofyzikální ústav
    KontaktJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Rok sběru2021
    Elektronická adresahttps://www.sciencedirect.com/science/article/abs/pii/S1572665720300370
Počet záznamů: 1