Počet záznamů: 1

Magnetic temperature-sensitive solid-lipid particles for targeting and killing tumor cells

SYSNO ASEP	0524157
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Magnetic temperature-sensitive solid-lipid particles for targeting and killing tumor cells
Tvůrce(i)	Swietek, Malgorzata Anna (UMCH-V)_{RID, ORCID} Panchuk, R. (UA) Skorokhyd, N. (UA) Černoch, Peter (UMCH-V)_{RID, ORCID} Finiuk, N. (UA) Klyuchivska, O. (UA) Hrubý, Martin (UMCH-V)_{RID, ORCID} Molčan, M. (SK) Berger, W. (AT) Trousil, Jiří (UMCH-V)_{RID, ORCID} Stoika, R. (UA) Horák, Daniel (UMCH-V)_{RID, ORCID}
Číslo článku	205
Zdroj.dok.	Frontiers in Chemistry. - : Frontiers Media - ISSN 2296-2646 Roč. 8, 9 April (2020), s. 1-18
Poč.str.	18 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	magnetic ; temperature sensitive ; solid lipid particles
Vědní obor RIV	CD - Makromolekulární chemie
Obor OECD	Polymer science
CEP	GA17-04918S GA ČR - Grantová agentura ČR
	GA18-07983S GA ČR - Grantová agentura ČR
	LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	UMCH-V - RVO:61389013
UT WOS	000529903600001
EID SCOPUS	85083878443
DOI	10.3389/fchem.2020.00205
Anotace	Magnetic and temperature-sensitive solid lipid particles (mag. SLPs) were prepared in the presence of oleic acid-coated iron oxide (IO-OA) nanoparticles with 1-tetradecanol and poly(ethylene oxide)-block-poly(ε-caprolactone) as lipid and stabilizing surfactant-like agents, respectively. The particles, typically ~850 nm in hydrodynamic size, showed heat dissipation under the applied alternating magnetic field. Cytotoxic activity of the mag.SLPs, non-magnetic SLPs, and iron oxide nanoparticles was compared concerning the mammalian cancer cell lines and their drug-resistant counterparts using trypan blue exclusion test and MTT assay. The mag.SLPs exhibited dose-dependent cytotoxicity against human leukemia cell lines growing in suspension (Jurkat and HL-60/wt), as well as the doxorubicin (Dox)- and vincristine-resistant HL-60 sublines. The mag.SLPs showed higher cytotoxicity toward drug-resistant sublines as compared to Dox. The human glioblastoma cell line U251 growing in a monolayer culture was also sensitive to mag.SLPs cytotoxicity. Staining of U251 cells with the fluorescent dyes Hoechst 33342 and propidium iodide (PI) revealed that mag.SLPs treatment resulted in an increased number of cells with condensed chromatin and/or fragmented nuclei as well as with blebbing of the plasma membranes. While the Hoechst 33342 staining of cell suggested the pro-apoptotic activity of the particles, the PI staining indicated the pro-necrotic changes in the target cells. These conclusions were confirmed by Western blot analysis of apoptosis-related proteins, study of DNA fragmentation (DNA laddering due to the inter-nucleosomal cleavage and DNA comets due to single strand breaks), as well as by FACS analysis of the patterns of cell cycle distribution (pre-G1 phase) and Annexin V/PI staining of the treated Jurkat cells. The induction of apoptosis or necrosis by the particles used to treat Jurkat cells depended on the dose of the particles. Production of the reactive oxygen species (ROS) was proposed as a potential mechanism of mag.SLPs-induced cytotoxicity. Accordingly, hydrogen peroxide and superoxide radical levels in mag.SLPs-treated Jurkat leukemic cells were increased by ~20–40 and ~70%, respectively. In contrast, the non-magnetic SLPs and neat iron oxides did not influence ROS levels significantly. Thus, the developed mag.SLPs can be used for effective killing of human tumor cells, including drug-resistant ones.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2021
Elektronická adresa	https://www.frontiersin.org/articles/10.3389/fchem.2020.00205/full

Počet záznamů: 1