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Magnetic temperature-sensitive solid-lipid particles for targeting and killing tumor cells

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    SYSNO ASEP0524157
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMagnetic temperature-sensitive solid-lipid particles for targeting and killing tumor cells
    Tvůrce(i) Swietek, Malgorzata Anna (UMCH-V) RID, ORCID
    Panchuk, R. (UA)
    Skorokhyd, N. (UA)
    Černoch, Peter (UMCH-V) RID, ORCID
    Finiuk, N. (UA)
    Klyuchivska, O. (UA)
    Hrubý, Martin (UMCH-V) RID, ORCID
    Molčan, M. (SK)
    Berger, W. (AT)
    Trousil, Jiří (UMCH-V) RID, ORCID
    Stoika, R. (UA)
    Horák, Daniel (UMCH-V) RID, ORCID
    Číslo článku205
    Zdroj.dok.Frontiers in Chemistry. - : Frontiers Research Foundation - ISSN 2296-2646
    Roč. 8, 9 April (2020), s. 1-18
    Poč.str.18 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovamagnetic ; temperature sensitive ; solid lipid particles
    Vědní obor RIVCD - Makromolekulární chemie
    Obor OECDPolymer science
    CEPGA17-04918S GA ČR - Grantová agentura ČR
    GA18-07983S GA ČR - Grantová agentura ČR
    LQ1604 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    ED1.1.00/02.0109 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaUMCH-V - RVO:61389013
    UT WOS000529903600001
    EID SCOPUS85083878443
    DOI10.3389/fchem.2020.00205
    AnotaceMagnetic and temperature-sensitive solid lipid particles (mag. SLPs) were prepared in the presence of oleic acid-coated iron oxide (IO-OA) nanoparticles with 1-tetradecanol and poly(ethylene oxide)-block-poly(ε-caprolactone) as lipid and stabilizing surfactant-like agents, respectively. The particles, typically ~850 nm in hydrodynamic size, showed heat dissipation under the applied alternating magnetic field. Cytotoxic activity of the mag.SLPs, non-magnetic SLPs, and iron oxide nanoparticles was compared concerning the mammalian cancer cell lines and their drug-resistant counterparts using trypan blue exclusion test and MTT assay. The mag.SLPs exhibited dose-dependent cytotoxicity against human leukemia cell lines growing in suspension (Jurkat and HL-60/wt), as well as the doxorubicin (Dox)- and vincristine-resistant HL-60 sublines. The mag.SLPs showed higher cytotoxicity toward drug-resistant sublines as compared to Dox. The human glioblastoma cell line U251 growing in a monolayer culture was also sensitive to mag.SLPs cytotoxicity. Staining of U251 cells with the fluorescent dyes Hoechst 33342 and propidium iodide (PI) revealed that mag.SLPs treatment resulted in an increased number of cells with condensed chromatin and/or fragmented nuclei as well as with blebbing of the plasma membranes. While the Hoechst 33342 staining of cell suggested the pro-apoptotic activity of the particles, the PI staining indicated the pro-necrotic changes in the target cells. These conclusions were confirmed by Western blot analysis of apoptosis-related proteins, study of DNA fragmentation (DNA laddering due to the inter-nucleosomal cleavage and DNA comets due to single strand breaks), as well as by FACS analysis of the patterns of cell cycle distribution (pre-G1 phase) and Annexin V/PI staining of the treated Jurkat cells. The induction of apoptosis or necrosis by the particles used to treat Jurkat cells depended on the dose of the particles. Production of the reactive oxygen species (ROS) was proposed as a potential mechanism of mag.SLPs-induced cytotoxicity. Accordingly, hydrogen peroxide and superoxide radical levels in mag.SLPs-treated Jurkat leukemic cells were increased by ~20–40 and ~70%, respectively. In contrast, the non-magnetic SLPs and neat iron oxides did not influence ROS levels significantly. Thus, the developed mag.SLPs can be used for effective killing of human tumor cells, including drug-resistant ones.
    PracovištěÚstav makromolekulární chemie
    KontaktEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Rok sběru2021
    Elektronická adresahttps://www.frontiersin.org/articles/10.3389/fchem.2020.00205/full
Počet záznamů: 1