Počet záznamů: 1  

Effects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes

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    SYSNO ASEP0523951
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEffects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes
    Tvůrce(i) Ostatná, Veronika (BFU-R) RID, ORCID
    West, R. M. (US)
    Celkový počet autorů2
    Číslo článku113884
    Zdroj.dok.Journal of Electroanalytical Chemistry - ISSN 1572-6657
    Roč. 860, 1.3.2020 (2020)
    Poč.str.7 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovahydrogen evolution ; proteins ; catalysis ; electrochemistry
    Vědní obor RIVAH - Ekonomie
    Obor OECDElectrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    CEPGA18-18154S GA ČR - Grantová agentura ČR
    Způsob publikováníOmezený přístup
    Institucionální podporaBFU-R - RVO:68081707
    UT WOS000519336100008
    EID SCOPUS85078696760
    DOI10.1016/j.jelechem.2020.113884
    AnotaceConstant current chronopotentiometric stripping (CPS) allows analysis of proteins based on measured peak H resulting from catalytic hydrogen evolution reaction. The technique is label-free and sensitive to the structure and stability of the protein adsorbed at the mercury electrode. Comparison of proteins must be carried out under the same conditions, including ionic strength, temperature, pH, and accumulation potential and time, as all of these factors can affect the protein stability and structure on the electrode surface. Here we show that for bovine serum albumin, uncontrolled disconnection of the cell after accumulation, as is necessary for ex situ CPS measurements, can cause an increased susceptibility to electric field-induced denaturation during the subsequent CPS measurement. This destabilization is attributed to oxidation of the Hg electrode during disconnection. For this reason, care much be taken when ex situ CPS measurement of protein is performed. (C) 2020 Elsevier B.V. All rights reserved.
    PracovištěBiofyzikální ústav
    KontaktJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Rok sběru2021
    Elektronická adresahttps://www.sciencedirect.com/science/article/abs/pii/S1572665720300679?via%3Dihub
Počet záznamů: 1