Počet záznamů: 1
Effects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes
- 1.
SYSNO ASEP 0523951 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of ex situ chronopotentiometric analysis on stability of bovine serum albumin on mercury electrodes Tvůrce(i) Ostatná, Veronika (BFU-R) RID, ORCID
West, R. M. (US)Celkový počet autorů 2 Číslo článku 113884 Zdroj.dok. Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 860, 1.3.2020 (2020)Poč.str. 7 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova hydrogen evolution ; proteins ; catalysis ; electrochemistry Vědní obor RIV AH - Ekonomie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA18-18154S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000519336100008 EID SCOPUS 85078696760 DOI 10.1016/j.jelechem.2020.113884 Anotace Constant current chronopotentiometric stripping (CPS) allows analysis of proteins based on measured peak H resulting from catalytic hydrogen evolution reaction. The technique is label-free and sensitive to the structure and stability of the protein adsorbed at the mercury electrode. Comparison of proteins must be carried out under the same conditions, including ionic strength, temperature, pH, and accumulation potential and time, as all of these factors can affect the protein stability and structure on the electrode surface. Here we show that for bovine serum albumin, uncontrolled disconnection of the cell after accumulation, as is necessary for ex situ CPS measurements, can cause an increased susceptibility to electric field-induced denaturation during the subsequent CPS measurement. This destabilization is attributed to oxidation of the Hg electrode during disconnection. For this reason, care much be taken when ex situ CPS measurement of protein is performed. (C) 2020 Elsevier B.V. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S1572665720300679?via%3Dihub
Počet záznamů: 1