Počet záznamů: 1  

A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms

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    SYSNO ASEP0523841
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevA new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms
    Tvůrce(i) Deschamps, A. (BE)
    Colinet, A. S. (BE)
    Zimmermannová, Olga (FGU-C) RID, ORCID
    Sychrová, Hana (FGU-C) RID, ORCID
    Morsomme, P. (BE)
    Číslo článku1881
    Zdroj.dok.Scientific Reports - ISSN 2045-2322
    Roč. 10, č. 1 (2020)
    Poč.str.11 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaGolgi ; pHluorin ; V-ATPase ; yeast ; STV1 ; VPH1 ; glucose availability
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGA17-01953S GA ČR - Grantová agentura ČR
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000515602400002
    EID SCOPUS85079069709
    DOI10.1038/s41598-020-58795-w
    AnotaceThe gradual acidification of the secretory pathway is conserved and extremely important for eukaryotic cells, but until now there was no pH sensor available to monitor the pH of the early Golgi apparatus in Saccharomyces cerevisiae. Therefore, we developed a pHluorin-based sensor for in vivo measurements in the lumen of the Golgi. By using this new tool we show that the cis- and medial-Golgi pH is equal to 6.6-6.7 in wild type cells during exponential phase. As expected, V-ATPase inactivation results in a near neutral Golgi pH. We also uncover that surprisingly Vph1p isoform of the V-ATPase is prevalent to Stv1p for Golgi acidification. Additionally, we observe that during changes of the cytosolic pH, the Golgi pH is kept relatively stable, mainly thanks to the V-ATPase. Eventually, this new probe will allow to better understand the mechanisms involved in the acidification and the pH control within the secretory pathway.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2021
    Elektronická adresahttps://doi.org/10.1038/s41598-020-58795-w
Počet záznamů: 1