Počet záznamů: 1

Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.)

SYSNO ASEP	0518420
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.)
Tvůrce(i)	Šimoníková, Denisa (UEB-Q)_ORCID Němečková, Alžběta (UEB-Q)_ORCID Karafiátová, Miroslava (UEB-Q)_{RID, ORCID} Uwimana, B. (UG) Swennen, R. (BE) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Hřibová, Eva (UEB-Q)_{RID, ORCID}
Celkový počet autorů	7
Číslo článku	1503
Zdroj.dok.	Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X Roč. 10, NOV 20 (2019)
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	repetitive dna-sequences ; flow-cytometric analysis ; ribosomal-rna genes ; 5s rdna ; fish ; hybridization ; wheat ; identification ; localization ; acuminata ; banana ; chromosome identification ; fluorescence in situ hybridization ; molecular karyotype ; Musa ; oligo painting FISH
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Agricultural biotechnology and food biotechnology
CEP	GA19-20303S GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	UEB-Q - RVO:61389030
UT WOS	000500980000001
EID SCOPUS	85076689140
DOI	10.3389/fpls.2019.01503
Anotace	Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis DH Pahang to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2020
Elektronická adresa	http://dx.doi.org/10.3389/fpls.2019.01503

Počet záznamů: 1