Počet záznamů: 1  

3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice

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    SYSNO ASEP0517438
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    Název3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice
    Tvůrce(i) Umek, N. (SI)
    Horvat, S. (SI)
    Cvetko, E. (SI)
    Kreft, M. (SI)
    Janáček, Jiří (FGU-C) RID, ORCID
    Kubínová, Lucie (FGU-C) RID, ORCID
    Pintaric, T.S. (SI)
    Eržen, I. (SI)
    Zdroj.dok.Histochemistry and Cell Biology - ISSN 0948-6143
    Roč. 152, č. 5 (2019), s. 323-331
    Poč.str.9 s.
    Jazyk dok.eng - angličtina
    Země vyd.DE - Německo
    Klíč. slovaobesity ; insulin resistence ; capillary network ; 3D analysis ; skeletal muscle ; fibre type
    Vědní obor RIVFP - Ostatní lékařské obory
    Obor OECDAnatomy and morphology (plant science to be 1.6)
    CEPLM2015062 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    EF16_013/0001775 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Výzkumná infrastrukturaCzech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i.
    Způsob publikováníOmezený přístup
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000495293400002
    EID SCOPUS85072132564
    DOI10.1007/s00418-019-01810-7
    AnotaceIn obesity, the skeletal muscle capillary network regresses and the insulin-mediated capillary recruitment is impaired. However, it has been shown that in the early stage of advanced obesity, an increased functional vascular response can partially compensate for other mechanisms of insulin resistance. The present study aimed to investigate the changes in the capillary network around individual muscle fibres during the early stage of obesity and insulin resistance in mice using 3D analysis. Capillaries and muscle fibres of the gluteus maximus muscles of seven high-fat-diet-induced obese and insulin-resistant mice and seven age-matched lean healthy mice were immunofluorescently labelled in thick transverse muscle sections. Stacks of images were acquired using confocal microscope. Capillary network characteristics were estimated by methods of quantitative image analysis. Muscle fibre typing was performed by histochemical analysis of myosin heavy chain isoforms on thin serial sections of skeletal muscle. Capillary length per muscle fibre length and capillary length per muscle fibre surface were increased by 27% and 23%, respectively, around small muscle fibres in obese mice, while there were no significant comparative differences around large fibres of obese and lean mice. Furthermore, the capillarization was larger around small compared to large fibres and there was a shift toward fast type myosin heavy chain isoforms, with no significant changes in muscle fibre diameters, tortuosity and anisotropy in obese mice. Overall, the results show that obese insulin-resistant mice have selective increase in capillarization around small predominantly intermediate muscle fibres, which is most likely related to the impaired glucose metabolism characteristic of type 2 diabetes.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2020
    Elektronická adresahttps://link.springer.com/article/10.1007%2Fs00418-019-01810-7
Počet záznamů: 1