Počet záznamů: 1
Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ]
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SYSNO ASEP 0504785 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Unbiased estimation of Norway spruce (Picea abies L. Karst.) chloroplast structure: Heterogeneity within needle mesophyll under different irradiance and [CO 2 ] Tvůrce(i) Kubínová, Z. (CZ)
Glanc, N. (CZ)
Radochová, Barbora (FGU-C) RID, ORCID
Lhotáková, Z. (CZ)
Janáček, Jiří (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCID
Albrechtová, J. (CZ)Zdroj.dok. Image Analysis and Stereology. - : Društvo za stereologijo in kvantitativno analizo slike - ISSN 1580-3139
Roč. 38, č. 1 (2019), s. 83-94Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. SI - Slovinsko Klíč. slova chloroplast number ; chloroplast ultrastructure ; elevated CO2 ; needle anatomy ; stereology Vědní obor RIV GK - Lesnictví Obor OECD Forestry CEP GAP501/10/0340 GA ČR - Grantová agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i.
CESNET II - 90042 - CESNET - zájmové sdružení právnických osobZpůsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000464195100009 EID SCOPUS 85065480947 DOI 10.5566/ias.2005 Anotace The main objective of this study was to find out whether the selected chloroplast characteristics measured in the mesophyll layer nearest to the needle surface (i.e., the first mesophyll layer) could be representative for the whole needle cross section. Two chloroplast sampling approaches were applied on Norway spruce needles during the investigation of the effects of different levels of air CO2 concentration and irradiance: (i) sampling only from the first mesophyll layer, and (ii) systematic uniform random (SUR) sampling. The selected characteristics were: (i) chloroplast area, (ii) starch grain area, and (iii) starch areal density on median chloroplast cross sections, and (iv) chloroplast number per unit of needle volume. It was shown that the first mesophyll layer was not representative for estimating all evaluated characteristics except the chloroplast area. Sampling only there caused obtaining slightly biased results, while SUR sampling gave unbiased estimations at the cost of longer measuring time. The major effect of studied factors was in starch areal density and starch grain area, which were larger in sun needles in elevated CO2 concentration in comparison with sun needles in ambient CO2 concentration. In conclusion, it was demonstrated that the first layer of mesophyll is not always representative for the needle cross section. If technically feasible, SUR is recommended for analysis of chloroplast ultrastructure. The simplified sampling design can be applied, e.g., for comparisons of many different treatments. However, it should be combined with other approaches to characterize the chloroplast function and the results carefully considered and interpreted. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://www.ias-iss.org/ojs/IAS/article/view/2005
Počet záznamů: 1