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Diverse roles of Tup1p and Cyc8p transcription regulators in the development of distinct types of yeast populations

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    SYSNO ASEP0504572
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevDiverse roles of Tup1p and Cyc8p transcription regulators in the development of distinct types of yeast populations
    Tvůrce(i) Váchová, Libuše (MBU-M) RID, ORCID
    Palková, Z. (CZ)
    Zdroj.dok.Current Genetics - ISSN 0172-8083
    Roč. 65, č. 1 (2019), s. 147-151
    Poč.str.5 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaYeast multicellular structures ; Colony biofilm ; Flocculation
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPLQ1604 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    ED1.1.00/02.0109 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000456958800019
    EID SCOPUS85052964454
    DOI10.1007/s00294-018-0883-z
    AnotaceYeasts create multicellular structures of varying complexity, such as more complex colonies and biofilms and less complex flocs, each of which develops via different mechanisms. Colony biofilms originate from one or more cells that, through growth and division, develop a complicated three-dimensional structure consisting of aerial parts, agar-embedded invasive parts and a central cavity, filled with extracellular matrix. In contrast, flocs arise relatively quickly by aggregation of planktonic cells growing in liquid cultures after they reach the appropriate growth phase and/or exhaust nutrients such as glucose. Creation of both types of structures is dependent on the presence of flocculins: Flo11p in the former case and Flo1p in the latter. We recently showed that formation of both types of structures by wild Saccharomyces cerevisiae strain BR-F is regulated via transcription regulators Tup1p and Cyc8p, but in a divergent manner. Biofilm formation is regulated by Cyc8p and Tup1p antagonistically: Cyc8p functions as a repressor of FLO11 gene expression and biofilm formation, whereas Tup1p counteracts the Cyc8p repressor function and positively regulates biofilm formation and Flo11p expression. In addition, Tup1p stabilizes Flo11p probably by repressing a gene coding for a cell wall or extracellular protease that is involved in Flo11p degradation. In contrast, formation of BR-F flocs is co-repressed by the Cyc8p-Tup1p complex. These findings point to different mechanisms involved in yeast multicellularity.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2020
    Elektronická adresahttps://link.springer.com/article/10.1007%2Fs00294-018-0883-z
Počet záznamů: 1