Počet záznamů: 1
Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins
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SYSNO ASEP 0504465 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins Tvůrce(i) Eckhardt, Adam (FGU-C) RID, ORCID
Novotný, T. (CZ)
Doubková, Martina (FGU-C) ORCID, RID
Hronková, Lucia (FGU-C)
Vajner, L. (CZ)
Pataridis, Statis (FGU-C)
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Kulhavá, Lucie (FGU-C) ORCID
Plencner, Martin (FGU-C) RID, ORCID
Knitlová, Jarmila (FGU-C) RID, ORCID
Lišková, Jana (FGU-C) RID, ORCID
Uhlík, J. (CZ)
Žaloudíková, M. (CZ)
Vondrášek, David (FGU-C) ORCID, SAI
Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Ošťádal, M. (CZ)Zdroj.dok. Journal of Orthopaedic Research. - : Wiley - ISSN 0736-0266
Roč. 37, č. 3 (2019), s. 769-778Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova clubfoot ; fibrosis, extracellular matrix ; proteomics ; collagen Vědní obor RIV FI - Traumatologie, ortopedie Obor OECD Orthopaedics CEP NV17-31564A GA MZd - Ministerstvo zdravotnictví Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000464419100026 EID SCOPUS 85061451671 DOI 10.1002/jor.24211 Anotace Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1002/jor.24211
Počet záznamů: 1