Počet záznamů: 1
The Coiled-Coil NLR Rph1, Confers Leaf Rust Resistance in Barley Cultivar Sudan
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SYSNO ASEP 0504182 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Coiled-Coil NLR Rph1, Confers Leaf Rust Resistance in Barley Cultivar Sudan Tvůrce(i) Dracatos, P. M. (AU)
Bartoš, Jan (UEB-Q) RID, ORCID
Elmansour, H. (AU)
Singh, D. (AU)
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Zhang, P. (AU)
Steuernagel, B. (GB)
Svačina, Radim (UEB-Q) ORCID
Cobbin, J. C.A. (AU)
Clark, B. (GB)
Hoxha, S. (AU)
Khatkar, M. S. (AU)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Wulff, B. B. H. (GB)
Park, R. F. (AU)Celkový počet autorů 15 Zdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 179, č. 4 (2019), s. 1362-1372Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova POWDERY MILDEW RESISTANCE ; RAPID GENE ISOLATION ; OF-FUNCTION MUTANTS Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000462993100015 EID SCOPUS 85064203800 DOI 10.1104/pp.18.01052 Anotace Unraveling and exploiting mechanisms of disease resistance in cereal crops is currently limited by their large repeat-rich genomes and the lack of genetic recombination or cultivar (cv)-specific sequence information. We cloned the first leaf rust resistance gene Rph1 (Rph1a) from cultivated barley (Hordeum vulgare) using “MutChromSeq,” a recently developed molecular genomics tool for the rapid cloning of genes in plants. Marker-trait association in the CI 9214/Stirling doubled haploid population mapped Rph1 to the short arm of chromosome 2H in a physical region of 1.3 megabases relative to the barley cv Morex reference assembly. A sodium azide mutant population in cv Sudan was generated and 10 mutants were confirmed by progeny-testing. Flow-sorted 2H chromosomes from Sudan (wild type) and six of the mutants were sequenced and compared to identify candidate genes for the Rph1 locus. MutChromSeq identified a single gene candidate encoding a coiled-coil nucleotide binding site Leucine-rich repeat (NLR) receptor protein that was altered in three different mutants. Further Sanger sequencing confirmed all three mutations and identified an additional two independent mutations within the same candidate gene. Phylogenetic analysis determined that Rph1 clustered separately from all previously cloned NLRs from the Triticeae and displayed highest sequence similarity (89%) with a homolog of the Arabidopsis (Arabidopsis thaliana) disease resistance protein 1 protein in Triticum urartu In this study we determined the molecular basis for Rph1-mediated resistance in cultivated barley enabling varietal improvement through diagnostic marker design, gene editing, and gene stacking technologies. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://doi.org/10.1104/pp.18.01052
Počet záznamů: 1