Počet záznamů: 1
Losartan treatment attenuates the development of neuropathic thermal hyperalgesia induced by peripheral nerve injury in rats
- 1.
SYSNO ASEP 0503875 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Losartan treatment attenuates the development of neuropathic thermal hyperalgesia induced by peripheral nerve injury in rats Tvůrce(i) Kalynovska, Nataliia (FGU-C) ORCID, RID
Diallo, Michael (FGU-C)
Paleček, Jiří (FGU-C) RID, ORCIDZdroj.dok. Life Sciences. - : Elsevier - ISSN 0024-3205
Roč. 220, Mar 1 (2019), s. 147-155Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova neuropathic pain ; neuroinflammation ; DRG ; Losartan ; SNL ; macrophage Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA18-09853S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000458400900016 EID SCOPUS 85061107249 DOI 10.1016/j.lfs.2019.02.008 Anotace Aims: Neuroinflammatory changes in the central nervous system are widely involved in the initiation and maintenance of neuropathic pain after peripheral nerve injury. The present study investigated how losartan treatment may affect the development of neuropathic pain and neuroinflammation. Main methods: The effect of losartan treatment on the development of peripheral neuropathy was studied in L5 spinal nerve ligation (SNL) model in rats with systemic (100 mg/kg) or intrathecal (10 mu l/20 mu M solution) application of losartan. Electronic von Frey filament and plantar test were used to determine pain thresholds to mechanical and thermal stimulations. At the 7th post-operative day, CD68-positive cells in DRG and dorsal roots were quantified by immunohistochemistry and western blot analyses were used to compare the expression levels of neuroinflammatory markers in lumbar spinal cord (SC). Key findings: Our data confirmed the presence of SNL-evoked heat hyperalgesia and mechanical allodynia. Losartan application blocked the SNL-induced hypersensitivity to thermal stimuli but failed to prevent mechanical allodynia. No significant difference between systemic and i.t. administration of losartan was observed. Immunohistochemistry confirmed the presence of infiltrated macrophages in the ipsilateral DRG that was significantly attenuated with the losartan treatment. Western blot SC tissue analysis revealed that systemic treatment with losartan prevented SNL-induced upregulation of CCR2, TNF alpha, TNFR1, and OX42 while its effect on CCL2 and AT1R expression was not significant. Significance: Our results show that losartan treatment attenuates neuroinflammation and neuropathic pain after SNL. These effects of losartan represent an interesting direction for the development of novel treatments of peripheral neuropathy. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1016/j.lfs.2019.02.008
Počet záznamů: 1