Počet záznamů: 1
Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity
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SYSNO ASEP 0502522 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Root enhancement in cytokinin-deficient oilseed rape causes leaf mineral enrichment, increases the chlorophyll concentration under nutrient limitation and enhances the phytoremediation capacity Tvůrce(i) Nehnevajova, E. (DE)
Ramireddy, E. (DE)
Stolz, A. (DE)
Gerdemann-Knörck, M. (DE)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Schmülling, T. (DE)Celkový počet autorů 7 Číslo článku 83 Zdroj.dok. BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 19, FEB 20 (2019)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Brassica napus ; Cytokinin ; Cytokinin oxidase/dehydrogenase ; Leaf mineral enrichment ; Phytoremediation ; Plant growth ; Root enhancement Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-07563S GA ČR - Grantová agentura ČR EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000459388100002 EID SCOPUS 85061964749 DOI 10.1186/s12870-019-1657-6 Anotace Background: Cytokinin is a negative regulator of root growth, and a reduction of the cytokinin content or signalling causes the formation a larger root system in model plants, improves their growth under drought and nutrient limitation and causes increased accumulation of elements in the shoot. Roots are an important but understudied target of plant breeding. Here we have therefore explored whether root enhancement by lowering the cytokinin content can also be achieved in oilseed rape (Brassica napus L.) plants. Results: Transgenic plants overexpressing the cKX2 gene of Arabidopsis thaliana encoding a cytokinin-degrading cytokinin oxidase/dehydrogenase showed higher cKX activity and a strongly reduced cytokinin content. Cytokinin deficiency led to the formation of a larger root system under different growth conditions, which was mainly due to an increased number of lateral and adventitious roots. In contrast, shoot growth was comparable to wild type, which caused an enhanced root-to-shoot ratio. Transgenic plants accumulated in their leaves higher concentrations of macro- And microelements including P, ca, Mg, S, Zn, cu, Mo and Mn. They formed more chlorophyll under Mg- And S-deficiency and accumulated a larger amount of cd and Zn from contaminated medium and soil. Conclusions: These findings demonstrate the usefulness of ectopic cKX gene expression to achieve root enhancement in oilseed rape and underpin the functional relevance of a larger root system. Furthermore, the lack of major developmental consequences on shoot growth in cytokinin-deficient oilseed rape indicates species-specific differences of cKX gene and/or cytokinin action. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1