Počet záznamů: 1
Cytotoxicity of hexahelicene and its effect on the aryl hydrocarbon receptor pathway.
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SYSNO ASEP 0502150 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytotoxicity of hexahelicene and its effect on the aryl hydrocarbon receptor pathway. Tvůrce(i) Vrba, J. (CZ)
Roubalová, L. (CZ)
Církva, Vladimír (UCHP-M) RID, ORCID, SAI
Storch, Jan (UCHP-M) RID, ORCID, SAI
Vacek, J. (CZ)Zdroj.dok. Toxicology in Vitro. - : Elsevier - ISSN 0887-2333
Roč. 57, June 2019 (2019), s. 105-109Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova PAH ; helicene ; biological activity Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP FV10082 GA MPO - Ministerstvo průmyslu a obchodu Způsob publikování Omezený přístup Institucionální podpora UCHP-M - RVO:67985858 UT WOS 000465050300013 EID SCOPUS 85062149211 DOI 10.1016/j.tiv.2019.02.020 Anotace Carbohelicenes are a group of helical-shaped polycyclic aromatic hydrocarbons. This study examined the effect of hexahelicene (or [6]helicene) and of its imidazolium derivative, 1-butyl-3-(2-methyl[6]helicenyl)-imidazolium bromide (I[6]H), on the activity of the aryl hydrocarbon receptor (AhR) and expression of cytochrome P450 1A1 (CYP1A1) in human hepatoma HepG2 cells. An MTT viability assay showed that both [6]helicene and I[6]H were cytotoxic to HepG2 cells after 24 h of exposure, with IC50 values of 0.9 and 8.4 μM, respectively. Using a gene reporter assay performed in transiently transfected HepG2 cells, we found that 1 μM [6]helicene, unlike I [6]H, significantly increased the activity of AhR to 2.1-fold compared to the control after 24 h of exposure. Moreover, [6]helicene induced a small but significant increase in the level of CYP1A1 mRNA. On the other hand, neither the protein level nor activity of CYP1A1 were affected by [6]helicene in HepG2 cells. The effect of [6] helicene on the AhR pathway was thus much lower than that of 2,3,7,8-tetrachlorodibenzo-p-dioxin, a potent AhR activator. We conclude that [6]helicene is a poor activator of the AhR pathway in HepG2 cells, and that the possible activation of the AhR pathway in vivo remains to be investigated. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2020 Elektronická adresa http://hdl.handle.net/11104/0294118
Počet záznamů: 1