Počet záznamů: 1

Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients

SYSNO ASEP	0493048
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Differential modulation of white adipose tissue endocannabinoid levels by n-3 fatty acids in obese mice and type 2 diabetic patients
Tvůrce(i)	Rossmeisl, Martin (FGU-C)_{RID, ORCID} Pavlišová, Jana (FGU-C) Janovská, Petra (FGU-C)_{RID, ORCID} Kuda, Ondřej (FGU-C)_{RID, ORCID, SAI} Bardová, Kristina (FGU-C)_{RID, ORCID, SAI} Hansíková, Jana (FGU-C) Svobodová, Michaela (FGU-C)_{RID, ORCID} Oseeva, Marina (FGU-C)_{ORCID, RID} Veleba, J. (CZ) Kopecký Jr., J. (CZ) Žáček, P. (CZ) Fišerová, E. (CZ) Pelikánová, T. (CZ) Kopecký, Jan (FGU-C)_{RID, ORCID}
Zdroj.dok.	Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. - : Elsevier - ISSN 1388-1981 Roč. 1863, č. 7 (2018), s. 712-725
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	2-AG ; DHEA ; high-fat diet ; obesity ; Omega-3 PUFA
Vědní obor RIV	FB - Endokrinologie, diabetologie, metabolizmus, výživa
Obor OECD	Endocrinology and metabolism (including diabetes, hormones)
CEP	GA14-09347S GA ČR - Grantová agentura ČR
	NV15-27431A GA MZd - Ministerstvo zdravotnictví
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000444364300005
EID SCOPUS	85045450551
DOI	10.1016/j.bbalip.2018.03.011
Anotace	n-3 polyunsaturated fatty acids (n-3 PUFA) might regulate metabolism by lowering endocannabinoid levels. We examined time-dependent changes in adipose tissue levels of endocannabinoids as well as in parameters of glucose homeostasis induced by n-3 PUFA in dietary-obese mice, and compared these results with the effect of n-3 PUFA intervention in type 2 diabetic (T2DM) subjects. Male C57BL/6J mice were fed for 8, 16 or 24 weeks a high-fat diet alone (cHF) or supplemented with n-3 PUFA (cHF + F). Overweight/obese, T2DM patients on metformin therapy were given for 24 weeks corn oil (Placebo, 5 g/day) or n-3 PUFA concentrate as above (Omega-3, 5 g/day). Endocannabinoids were measured by liquid chromatography-tandem mass-spectrometry. Compared to cHF-fed controls, the cHF + F mice consistently reduced 2-arachidonoylglycerol (up to similar to 2-fold at week 24) and anandamide (similar to 2-fold) in adipose tissue, while the levels of endocannabinoid-related anti-inflammatory molecules N-eicosapentaenoyl ethanolamine (EPEA) and N-docosahexaenoyl ethanolamine (DHEA) increased more than similar to 10-fold and similar to 8-fold, respectively. At week 24, the cHF + F mice improved glucose tolerance and fasting blood glucose, the latter being positively correlated with adipose 2-arachidonoylglycerol levels only in obese cHF-fed controls, like fasting insulin and HOMA-IR. In the patients, n-3 PUFA failed to reduce 2-arachidonoylglycerol and anandamide levels in adipose tissue and serum, but they increased both adipose tissue and serum levels of EPEA and DHEA. In conclusion, the inability of n-3 PUFA to reduce adipose tissue and serum levels of classical endocannabinoids might contribute to a lack of beneficial effects of these lipids on glucose homeostasis in T2DM patients.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2019

Počet záznamů: 1