Počet záznamů: 1
Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins
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SYSNO ASEP 0492202 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins Tvůrce(i) Kubiasová, K. (CZ)
Mik, V. (CZ)
Nisler, Jaroslav (UEB-Q) RID, ORCID
Hönig, Martin (UEB-Q) RID, ORCID
Husičková, A. (CZ)
Spíchal, L. (CZ)
Pěkná, Z. (CZ)
Šamajová, O. (CZ)
Doležal, Karel (UEB-Q) RID, ORCID
Plíhal, O. (CZ)
Benková, E. (AT)
Strnad, Miroslav (UEB-Q) RID, ORCID
Plíhalová, Lucie (UEB-Q) ORCID, RIDCelkový počet autorů 13 Zdroj.dok. Phytochemistry. - : Elsevier - ISSN 0031-9422
Roč. 150, JUN (2018), s. 1-11Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova barley aleurone protoplasts ; endoplasmic-reticulum ; nodule organogenesis ; histidine kinase ; arabidopsis ; receptor ; binding ; probe ; ahk4 ; identification ; 6-[(3-methylbut-2-en-1-yl)amino]purine ; N-6-isopentenyladenine ; Isoprenoid ; Cytokinin ; Fluorescent label ; Fluorescent probe ; Linker ; Competitive receptor bioassay ; arr5:gus ; Live cell confocal microscopy Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Plant sciences, botany CEP LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA16-04184S GA ČR - Grantová agentura ČR GAP501/10/1450 GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000435623400001 EID SCOPUS 85042918796 DOI 10.1016/j.phytochem.2018.02.015 Anotace Isoprenoid cytokinins play a number of crucial roles in the regulation of plant growth and development. To study cytokinin receptor properties in plants, we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine (N-6-isopentenyladenine, iP) with several fluorescent labels attached to the C2 or N9 atom of the purine moiety via a 2-or 6-carbon linker. The fluorescent labels included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B (RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5). All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels to iP via the linkers mostly disrupted binding to the receptor, several fluorescent derivatives interacted well. For this reason, three derivatives, two rhodamine B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that the three derivatives were able to activate transcription of cytokinin response regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and 4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative controls (19, 20 and 29, respectively) were used for in planta staining experiments in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1