Počet záznamů: 1  

Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

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    SYSNO ASEP0489869
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevPhytase-mediated enzymatic mineralization of chitosan-enriched hydrogels
    Tvůrce(i) Lišková, Jana (FGU-C) RID, ORCID
    Douglas, T.E.L. (BE)
    Wijnants, R. (BE)
    Samal, S. K. (BE)
    Mendez, A. C. (DK)
    Chronakis, I. (DK)
    Bačáková, Lucie (FGU-C) RID, ORCID
    Skirtach, A. G. (BE)
    Zdroj.dok.Materials Letters. - : ELSEVIER SCIENCE BV - ISSN 0167-577X
    Roč. 214, Mar 1 (2018), s. 186-189
    Poč.str.4 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovabiomaterials ; biomimetic ; composite materials
    Vědní obor RIVEI - Biotechnologie a bionika
    Obor OECDTechnologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics)
    CEPNV15-33018A GA MZd - Ministerstvo zdravotnictví
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000419724400048
    EID SCOPUS85042236906
    DOI10.1016/j.matlet.2017.12.004
    AnotaceHydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2019
Počet záznamů: 1